说明：生化研究，能使过氧化氢分解为水和氧

过氧化氢酶 来源于牛肝脏

Catalase from bovine liver

lyophilized powder, 2,000-5,000 units/mg protein

Frequently Asked Questions

Live Chat d Frequently Asked Questions are available for this Product.

Components

Catalase from bovine liver is a tetramer consisting of 4 equal subunits each with a 60 kDa molecular weight. Each of these subunits contains iron bound to a protoheme IX group. The enzyme will also strongly bind to NADP, where NADP d the heme group are within 13.7 gstroms.

Application

Catalase acts as a natural tioxidt to study the roles of reactive oxygen species in gene expression d apoptosis. It has also been used to protect against oxidative damage to proteins, lipids, d nucleic acids. Industrially, catalses have been used to remove hydrogen peroxide added to milk d cheese, in textile bleaching, d to examine its positive effects on the viability of DNA-repair mutts of E. coli.

Biochem/physiol Actions

激活过氧化氢的分解，用于研究活性氧在基因表达和细胞凋亡中的作用的天然抗氧化剂。

This product doesn′t need y activators, but it is inhibited by 3-amino-1-H-1,2,4 triazole, cyide, azide, hydroxylamine, cyogens bromide, 2-mercaptoethol, dithiothreitol, diisidnie d nitrate.

Caution

不建议冷冻溶液。

Preparation Note

由缩二脲测定的蛋白质

Unit Definition

在 pH 7.0 和 25°C 下，一单位本品每分钟可以分解 1.0μmol H2O2，同时 H2O2 浓度从 10.3mM 降至 9.2mM，这是通过 A240 的降低速率来测量的。

Related Categories 1.11.1.6 Catalase, 1.x.x.x Oxidoreductases, Application Index, Diagnostic d Analytical Enzymes, Enzyme Class Index,

General Metabolic Enzymes, General Metabolic Enzymes A-H, 代谢组学, 生化试剂, 细胞生物学, 酶、抑制剂和底物

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Quality Level ELITE

form lyophilized powder

mol wt tetramer mol wt ~250 kDa

isoelectric point 5.4

storage temp. −20°C

Gene Information cow … CAT(280743)