Mouse anti-MGMT Monoclonal Antibody(1527CT158.87.44)描述别名宿主特异性反应种属应用分子量类型克隆号同种型储存/保存方法研究领域背景说明细胞定位UniProt参考文献
| 概述 | |
| 描述 |
Purified Mouse Monoclonal Antibody (Mab)
|
| 别名 |
MGMT抗体;Methylated-DNA–protein-cysteine methyltransferase; 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase
|
| 宿主 |
Mouse
|
| 特异性 |
This MGMT antibody is generated from a mouse immunized with a recombinant protein.
|
| 反应种属 |
Human
|
| 应用 |
FC~~1:25
WB~~1:1000-1:2000 |
| 分子量 |
Predicted molecular weight: 22kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 性能 | |
| 类型 |
Monoclonal Antibody
|
| 克隆号 |
1527CT158.87.44
|
| 同种型 |
IgG1,k
|
| 储存/保存方法 |
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
|
| 研究领域 |
Others
|
| 靶标 | |
| 背景说明 |
Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
|
| 细胞定位 |
Nucleus.
|
| UniProt |
P16455
|
| 参考文献 | |
| 参考文献 |
Tano K.,et al.Proc. Natl. Acad. Sci. U.S.A. 87:686-690(1990).
Rydberg B.,et al.J. Biol. Chem. 265:9563-9569(1990). Koike G.,et al.J. Biol. Chem. 265:14754-14762(1990). Hayakawa H.,et al.J. Mol. Biol. 213:739-747(1990). Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases. |
实验结果图
All lanes : Anti-MGMT Antibody at1:1000-1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: MOLT-4 whole cell lysate Lane 4: Raji whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST.