Recombinant SARS-CoV-2(Omicron/BA.2.75) Spike Protein(C-10His)种属来源分子量外观形态序列标签纯度内毒素水平标记溶解方法储存/保存方法背景说明Accession
| 概述 | |
| 种属 |
Virus
|
| 来源 |
HEK 293
|
| 分子量 |
Detects a band of approximately 150 kD(Predicted molecular weight: 134.4 kD)
|
| 外观 |
Lyophilized Powder
|
| 形态 |
Lyophilized from a 0.2 μm filtered solution of 0.2M PBS, pH7.4.
|
| 性能 | |
| 序列 |
Val16-Pro1213
VNLITRTQSYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLDVYYHENNKSRMESELRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPVNLGRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSSWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFHEVFNATRFASVYAWNRKRISNCVADYSVLYNFAPFFAFKCYGVSPTKLNDLCFTNVYADSFVIRGNEVSQIAPGQTGNIADYNYKLPDDFTGCVIAWNSNKLDSKVSGNYNYLYRLFRKSKLKPFERDISTEIYQAGNKPCNGVAGFNCYFPLQSYGFRPTYGVGHQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQGVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEYVNNSYECDIPIGAGICASYQTQTKSHRAAASVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLKRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKYFGGFNFSQILPDPSKPSKRSPIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGPALQIPFPMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTPSALGKLQDVVNHNAQALNTLVKQLSSKFGAISSVLNDILSRLDPPEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPGGGGSHHHHHHHHHH |
| 标签 |
C-10His
|
| 纯度 |
＞95% by SDS-PAGE
|
| 内毒素水平 |
< 1EU/ug
|
| 标记 |
unconjugated
|
| 溶解方法 |
Centrifuge tubes before opening.Reconstitute no more than 1 mg/mL according to the size in deionized water.
|
| 储存/保存方法 |
Within 1 month, 2 to 8 °C under sterile conditions after reconstitution. 12 months from date of receipt, -20 to -110 °C as supplied. Avoid repeated freeze-thaw cycles.
|
| 靶标 | |
| 背景说明 |
Sars-cov-2 (2019-NCOV) infects human respiratory epithelial cells through binding to human ACE2 receptors. Spike protein is a large type I transmembrane protein containing two subunits S1 and S2.S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing cell surface receptors. S2 contains the basic elements required for membrane fusion. S protein plays a key role in inducing neutralizing antibody and T cell responses as well as protective immunity.
|
| Accession |
P0DTC2
|
实验结果图
2μg（R: reducing conditions）
Immobilized SARS-CoV-2 (BA.2.75/Omicron) Spike protein, His Tag at 4 μg/mL (50 μL/well) can bind Human ACE2, hFc tag with EC50 of 7.860-12.68 ng/ml.