Rabbit anti-Hemoglobin Recombinant Monoclonal Antibody(193-71)别名宿主反应种属应用免疫原形式浓度纯化方法类型克隆号储存/保存方法存储溶液背景说明细胞定位UniProt
| 概述 | |
| 别名 |
Hb
|
| 宿主 |
Rabbit
|
| 反应种属 |
Human
|
| 应用 |
WB: 1:500, IP: 1:25, IHC-P: 1:5000
|
| 免疫原 |
Native protein
|
| 性能 | |
| 形式 |
Liquid
|
| 浓度 |
0.25 mg/ml
|
| 纯化方法 |
Protein A affinity column
|
| 类型 |
Monoclonal Antibody
|
| 克隆号 |
193-71
|
| 储存/保存方法 |
Store at -20℃ for one year.
|
| 存储溶液 |
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
|
| 靶标 | |
| 背景说明 |
The primary function of Hemoglobin (Hb) is to transport oxygen (O2) from the lung to tissues, binding and
releasing O2 in a cooperative manner [PMID: 32189307]. Hemoglobin is a tetramer, consisting of two α-subunits (α1 and α2) and two β subunits (β1 and β2) that are structurally similar and about the same size. The two αβ dimers (named α1β1 and α2β2) are arranged around a 2-fold axis of symmetry resulting in a large central water cavity in the T or unliganded or deoxygenated structure and a narrower cavity in the R or liganded or oxygenated structure. |
| 细胞定位 |
Blood microparticle, Cytosol
|
| UniProt |
P69905, P68871
|
实验结果图
WB result of Hemoglobin Rabbit mAb Primary antibody: Hemoglobin Rabbit mAb at 1/500 dilution Lane 1: 293T whole cell lysate 20 µg Lane 2: TF-1 whole cell lysate 20 µg Negative control: 293T whole cell lysate Secondary antibody: #JP20040 at 1/10000 dilution Predicted MW: 13 kDa Observed MW: 13 kDa Exposure time: 120s
IHC shows positive staining in paraffin-embedded human tonsil. Anti-Hemoglobin antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human placenta. Anti-Hemoglobin antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostate. Anti-Hemoglobin antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-Hemoglobin antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-Hemoglobin antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Hemoglobin Rabbit mAb at 1/25 dilution (1µg) immunoprecipitating Hemoglobin in 0.4mg TF-1 whole cell lysate. Western blot was performed on the immunoprecipitate using Hemoglobin Rabbit mAb at 1/1000 dilution. Secondary antibody (HRP) for IP was used at 1/400 dilution. Lane 1 : TF-1 whole cell lysate 20µg(input) Lane 2 : Hemoglobin Rabbit mAb IP in TF-1 whole cell lysate Lane 3 : Rabbit monoclonal IgG IP in TF-1 whole cell lysate Predicted MW: 13 kDa Observed MW: 13 kDa Exposure time: 60s