Rabbit anti-S6 Ribosomal Protein Recombinant Monoclonal Antibody(S-479-1)别名宿主反应种属应用免疫原形式浓度纯化方法类型克隆号同种型储存/保存方法存储溶液背景说明UniProt
| 概述 | |
| 别名 |
Small ribosomal subunit protein eS6; 40S ribosomal protein S6; Phosphoprotein NP33; RPS6
|
| 宿主 |
Rabbit
|
| 反应种属 |
Human, Mouse, Rat
|
| 应用 |
WB: 1:1000, IP: 1:50, IHC-P: 1:500
|
| 免疫原 |
Synthetic peptide
|
| 性能 | |
| 形式 |
Liquid
|
| 浓度 |
0.5 mg/mL
|
| 纯化方法 |
Protein A affinity column
|
| 类型 |
Monoclonal Antibody
|
| 克隆号 |
S-479-1
|
| 同种型 |
IgG
|
| 储存/保存方法 |
Store at -20℃ for one year.
|
| 存储溶液 |
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
|
| 靶标 | |
| 背景说明 |
Ribosomal protein S6 (rpS6 or eS6) is a component of the 40S ribosomal subunit and is therefore involved in translation. Mouse model studies have shown that phosphorylation of eS6 is involved in the regulation of cell size, cell proliferation, and glucose homeostasis. tudies show that the p70 ribosomal protein S6 kinases (S6K1 and S6K2) and p90 ribosomal protein S6 kinases (RSK) both phosphorylate eS6 and that S6K1 and S6K2 predominate this function. Pathways leading to the induction of human eS6 phosphorylation have been found to enhance IL-8 protein synthesis. This mechanism is dependent on A/U-rich proximal sequences (APS) found in the 3’UTR of IL-8 immediately after the stop codon.
|
| UniProt |
P62753
|
实验结果图
WB result of S6 Ribosomal Protein Rabbit mAb Primary antibody: S6 Ribosomal Protein Rabbit mAb at 1/1000 dilution Lane 1: HeLa whole cell lysate 20 ug Lane 2: MCF7 whole cell lysate 20 ug Lane 3: HepG2 whole cell lysate 20 ug Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 29 kDa Observed MW: 29 kDa Exposure time: 180 s
WB result of S6 Ribosomal Protein Rabbit mAb Primary antibody: S6 Ribosomal Protein Rabbit mAb at 1/1000 dilution Lane 1: NIH/3T3 whole cell lysate 20 ug Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 29 kDa Observed MW: 29 kDa Exposure time: 180 s
WB result of S6 Ribosomal Protein Rabbit mAb Primary antibody: S6 Ribosomal Protein Rabbit mAb at 1/1000 dilution Lane 1: PC-12 whole cell lysate 20 ug Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 29 kDa Observed MW: 29 kDa Exposure time: 180 s
S6 Ribosomal Protein Rabbit mAb at 1/50 dilution (1 ug) immunoprecipitating S6 Ribosomal Protein in 0.4 mg HeLa whole cell lysate. Western blot was performed on the immunoprecipitate using S6 Ribosomal Protein Rabbit mAb at 1/1000 dilution. Secondary antibody (HRP) for IP was used at 1/400 dilution. Lane 1: HeLa whole cell lysate 20 ug (Input) Lane 2: S6 Ribosomal Protein Rabbit mAb IP in HeLa whole cell lysate Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate Predicted MW: 29 kDa Observed MW: 29 kDa Exposure time: 150 s
IHC shows positive staining in paraffin-embedded human tonsil. Anti-S6 Ribosomal Protein antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-S6 Ribosomal Protein antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human diffuse large B-cell lymphoma. Anti-S6 Ribosomal Protein antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebellum. Anti-S6 Ribosomal Protein antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebellum. Anti-S6 Ribosomal Protein antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.