Rabbit anti-DNMT3A Polyclonal Antibody(Center R478)别名宿主特异性反应种属应用分子量类型同种型储存/保存方法存储溶液研究领域背景说明细胞定位UniProt参考文献
| 概述 | |
| 别名 |
Dnmt3A抗体, Dnmt3A2抗体;DNA (cytosine-5)-methyltransferase 3A; Dnmt3a; DNA methyltransferase HsaIIIA; DNA MTase HsaIIIA; MHsaIIIA; DNMT3A
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| 宿主 |
Rabbit
|
| 特异性 |
This DNMT3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 457-486 amino acids from the Central region of human DNMT3A.
|
| 反应种属 |
Human
|
| 应用 |
WB~~1:1000
IHC-P~~1:10~50 |
| 分子量 |
Predicted molecular weight: 102kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 性能 | |
| 类型 |
Polyclonal Antibody
|
| 同种型 |
Rabbit Ig
|
| 储存/保存方法 |
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
|
| 存储溶液 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
|
| 研究领域 |
Chromatin
|
| 靶标 | |
| 背景说明 |
Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated ‘Lys-36’ of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity.
|
| 细胞定位 |
Nucleus. Cytoplasm. Note=Accumulates in the major satellite repeats at pericentric heterochromatin
|
| UniProt |
Q9Y6K1
|
| 参考文献 | |
| 参考文献 |
Holz-Schietinger, C., et al. J. Biol. Chem. 285(38):29091-29100(2010) Kelemen, L.E., et al. Cancer Epidemiol. Biomarkers Prev. 19(7):1822-1830(2010) Park, C.W., et al. J Cardiovasc Transl Res 3(3):290-295(2010) Haggarty, P., et al. PLoS ONE 5 (6), E11329 (2010) : Zhao, Z., et al. J. Biomed. Biotechnol. 2010, 737535 (2010) :
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实验结果图
Anti-DNMT3A Antibody (R478WMutant) at 1:1000 dilution + U-2OS whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 102 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
DNMT3A Antibody (Center R478)(Cat. #AP14633c) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the DNMT3A antibody detected the DNMT3A protein (arrow).
DNMT3A Antibody (Center R478WMutant) (AP14633c)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DNMT3A Antibody (Center R478WMutant) for immunohistochemistry. Clinical relevance has not been evaluated.