Rabbit anti-PMEL/Melanoma gp100 Recombinant Monoclonal Antibody(434-70)别名宿主反应种属应用免疫原形式浓度纯化方法类型克隆号储存/保存方法存储溶液背景说明细胞定位UniProt
| 概述 | |
| 别名 |
ME20-M; ME20M; Pmel 17; P1; P100; Premelanosome protein; Silver locus protein homolog; Melanosome
|
| 宿主 |
Rabbit
|
| 反应种属 |
Human
|
| 应用 |
WB: 1:1000, IHC-P: 1:1000
|
| 免疫原 |
Synthetic Peptide
|
| 性能 | |
| 形式 |
Liquid
|
| 浓度 |
0.25 mg/mL
|
| 纯化方法 |
Protein A affinity column
|
| 类型 |
Monoclonal Antibody
|
| 克隆号 |
434-70
|
| 储存/保存方法 |
Store at -20℃ for one year.
|
| 存储溶液 |
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
|
| 靶标 | |
| 背景说明 |
PMEL encodes a key component of the melanosome, the organelle essential for melanin synthesis, storage and transport [PMID: 30561643]. Melanoma gp100 is a protein highly expressed in melanoma tissue.
|
| 细胞定位 |
Melanosome, Endoplasmic reticulum membrane, Endosome, Secreted
|
| UniProt |
P40967
|
实验结果图
WB result of PMEL/Melanoma gp100 Rabbit mAb Primary antibody: PMEL/Melanoma gp100 Rabbit mAb at 1/1000 dilution Lane 1: HeLa whole cell lysate 20 ug Lane 2: SK-MEL-28 whole cell lysate 20 ug Negative control: HeLa whole cell lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 70 kDa Observed MW: 100,26 kDa Exposure time: 13 s
IHC shows positive staining in paraffin-embedded human melanoma. Anti-PMEL/Melanoma gp100 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human colon cancer. Anti- PMEL/Melanoma gp100 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human transitional cell carcinoma. Anti- PMEL/Melanoma gp100 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human prostatic carcinoma. Anti- PMEL/Melanoma gp100 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.