Rabbit anti-CD49b Recombinant Monoclonal Antibody(399-52)别名宿主反应种属应用免疫原形式浓度纯化方法类型克隆号储存/保存方法存储溶液背景说明细胞定位UniProt
| 概述 | |
| 别名 |
Integrin α2; CD49 antigen-like family member B; Collagen receptor; GPIa; VLA-2 subunit alpha; ITGA2
|
| 宿主 |
Rabbit
|
| 反应种属 |
Human
|
| 应用 |
WB: 1:1000, IHC-P: 1:500
|
| 免疫原 |
Synthetic peptide
|
| 性能 | |
| 形式 |
Liquid
|
| 浓度 |
0.5 mg/mL
|
| 纯化方法 |
Protein A affinity column
|
| 类型 |
Monoclonal Antibody
|
| 克隆号 |
399-52
|
| 储存/保存方法 |
Store at -20℃ for one year.
|
| 存储溶液 |
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
|
| 靶标 | |
| 背景说明 |
Integrin alpha 2, also known as VLA-2, GPIa-IIa, CD49b, was first identified as an extracellular matrix receptor for collagens and/or laminins. It is now recognized that the Integrin alpha 2 serves as a receptor for many matrix and nonmatrix molecules. Extensive analyses have clearly elucidated the α2 I domain structural motifs required for ligand binding, and also defined distinct conformations that lead to inactive, partially active or highly active ligand binding. The mechanisms by which the Integrin alpha 2 plays a critical role in platelet function and homeostasis have been carefully defined via in vitro and in vivo experiments. Genetic and epidemiologic studies have confirmed human physiology and disease states mediated by this receptor in immunity, cancer, and development [PMID: 25023166].
|
| 细胞定位 |
Membrane
|
| UniProt |
P17301
|
实验结果图
WB result of CD49b Rabbit mAb Primary antibody: CD49b Rabbit mAb at 1/1000 dilution Lane 1: A549 whole cell lysate 20 µg Lane 2: A431 whole cell lysate 20 µg Lane 3: T-47D whole cell lysate 20 µg Lane 4: PC-3 whole cell lysate 20 µg Lane 5: MCF7 whole cell lysate 20 µg Secondary antibody: #JP20040 at 1/10000 dilution Predicted MW: 129kDa Observed MW: 150kDa Exposure time: 90s
IHC shows positive staining in paraffin-embedded human colon. Anti-CD49b / Integrin alpha 2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-CD49b / Integrin alpha 2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-CD49b / Integrin alpha 2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-CD49b / Integrin alpha 2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-CD49b / Integrin alpha 2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.