Ig gamma-2 chain C region; IgG2B Fc

Mouse

HEK293

32-35 kDa(Reducing)

Lyophilized Powder

Lyophilized from a 0.22 μm filtered solution of PBS, pH7.4.

Glu97-Lys335

＞95% by SDS-PAGE&SEC-HPLC

＜0.1 EU/μg(gel-clot)

unconjugated

Centrifuge tubes before opening.Reconstitute no more than 1 mg/mL according to the size in deionized water.

Within 1 month, 2 to 8 °C under sterile conditions after reconstitution. 12 months from date of receipt, -20 to -80 °C as supplied. Avoid repeated freeze-thaw cycles.

Immunoglobulin G (IgG) is the major antibody component in normal human serum, produced and secreted by B cells, and is a molecule with a molecular weight of approximately 150 kDa. IgG contains two heavy chains (50 kDa) and two light chains (25 kDa) linked by disulfide bonds. After pepsin cleavage, IgG is divided into F(ab)s with an antigen-binding site and a highly conserved Fc segment. The Fc segment has a highly conserved n-glycosylation site. There are 5 subtypes of IgG in mice (IgG1, IgG2a, IgG2b, IgG2c and IgG3) and 4 in rat (IgG1, IgG2a, IgG2b and IgG2c). Studies have found that IgG2a is superior to IgG1 in activating complement. IgG2 is mainly used to neutralize antigens or block the binding of receptor ligands. In addition, human IgG2 EGFR antibodies can mediate ADCC effects through myeloid cells. This product is made from HEK293 cell line recombinantly expressing the Fc fragment of mouse-derived IgG2b, which is purified, sterilized, filtered, packaged and lyophilized.

P01867-2