Rabbit anti-Human IgG3 F(c) Recombinant Monoclonal Antibody(318-77)别名宿主反应种属应用免疫原形式浓度纯化方法类型克隆号储存/保存方法存储溶液背景说明细胞定位UniProt
| 概述 | |
| 别名 |
Immunoglobulin heavy constant gamma 3, HDC, Heavy chain disease protein, Ig gamma-3 chain C region, IGHG3
|
| 宿主 |
Rabbit
|
| 反应种属 |
Human
|
| 应用 |
WB: 1:1000-1:20000, IHC-P: 1:5000
|
| 免疫原 |
Native protein
|
| 性能 | |
| 形式 |
Liquid
|
| 浓度 |
0.5 mg/mL
|
| 纯化方法 |
Protein A affinity column
|
| 类型 |
Monoclonal Antibody
|
| 克隆号 |
318-77
|
| 储存/保存方法 |
Store at -20℃ for one year.
|
| 存储溶液 |
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
|
| 靶标 | |
| 背景说明 |
The intact antibody of human immunoglobulin (IgG) is composed of the fragment for antigen binding (Fab) and the crystallizable fragment (Fc) for binding of Fcγ receptors. Among the four subclasses of human IgG (IgG1, IgG2, IgG3, IgG4), which differ in their constant regions, particularly in their hinges and CH2 domains, IgG1 has the highest FcγR-binding affinity, followed by IgG3, IgG2, and IgG4 [PMID: 32370812].
|
| 细胞定位 |
Secreted, Cell membrane
|
| UniProt |
P01860
|
实验结果图
WB result of Human IgG3 Rabbit mAb Primary antibody: Human IgG3 Rabbit mAb at 1/1000 dilution Lane 1: IgG1 FC recombinant protein lysate 10 ng Lane 2: IgG2 FC recombinant protein lysate 10 ng Lane 3: IgG3 FC recombinant protein lysate 10 ng Lane 4: IgG4 FC recombinant protein lysate 10 ng Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: IgG1 FC、IgG2 FC、IgG4 FC: 27 kDa; IgG3 FC: 33 kDa Observed MW: IgG3 FC: 36 kDa Exposure time: 10s
WB result of Human IgG3 Rabbit mAb Primary antibody: Human IgG3 Rabbit mAb at 1/20000 dilution Lane 1: Human serum lysate 5 μg Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 36 kDa Observed MW: 58 kDa Exposure time: 180s
IHC shows positive staining in paraffin-embedded human tonsil. Anti-Human IgG3 antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.