Mouse anti-CASP3 Monoclonal Antibody(1262CT521.280.101)描述别名宿主特异性反应种属应用分子量类型克隆号同种型储存/保存方法存储溶液研究领域背景说明细胞定位UniProt参考文献
概述 | |
描述 |
Mouse Monoclonal Antibody (Mab)
|
别名 |
CASP3抗体;Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1; Caspase-3 subunit p17; Caspase-3 subunit p12; CASP3; CPP32
|
宿主 |
Mouse
|
特异性 |
Purified His-tagged CASP3 protein was used to produced this monoclonal antibody.
|
反应种属 |
Human
|
应用 |
WB~~1:2000
|
分子量 |
Predicted molecular weight: 32kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
性能 | |
类型 |
Monoclonal Antibody
|
克隆号 |
1262CT521.280.101
|
同种型 |
IgG1
|
储存/保存方法 |
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
|
存储溶液 |
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
|
研究领域 |
Cancer;Cell Biology;Metabolism;Neuroscience;Amyotrophic Lateral Sclerosis ALS
|
靶标 | |
背景说明 |
Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a ‘216-Asp-|-Gly-217’ bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
|
细胞定位 |
Cytoplasm.
|
UniProt |
P42574
|
参考文献 | |
参考文献 |
Choudhary C., et al. Science 325:834-840(2009).
Fernandes-Alnemri T., et al. J. Biol. Chem. 269:30761-30764(1994). Tewari M., et al. Cell 81:801-809(1995). Pelletier M., et al. Biochem. Biophys. Res. Commun. 316:93-99(2004). Ota T., et al. Nat. Genet. 36:40-45(2004). |
实验结果图
CASP3 Antibody (Cat. #JP100394) western blot analysis in Jurkat,Ramos cell line lysates (35μg/lane).This demonstrates the CASP3 antibody detected the CASP3 protein (arrow).