Mouse anti-CASP3 Monoclonal Antibody(1262CT521.280.101)

Mouse anti-CASP3 Monoclonal Antibody(1262CT521.280.101)描述别名宿主特异性反应种属应用分子量类型克隆号同种型储存/保存方法存储溶液研究领域背景说明细胞定位UniProt参考文献

概述
描述
Mouse Monoclonal Antibody (Mab)
别名
CASP3抗体;Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1; Caspase-3 subunit p17; Caspase-3 subunit p12; CASP3; CPP32
宿主
Mouse
特异性
Purified His-tagged CASP3 protein was used to produced this monoclonal antibody.
反应种属
Human
应用
WB~~1:2000
分子量
Predicted molecular weight: 32kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
性能
类型
Monoclonal Antibody
克隆号
1262CT521.280.101
同种型
IgG1
储存/保存方法
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
存储溶液
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
研究领域
Cancer;Cell Biology;Metabolism;Neuroscience;Amyotrophic Lateral Sclerosis ALS
靶标
背景说明
Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a ‘216-Asp-|-Gly-217’ bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
细胞定位
Cytoplasm.
UniProt
P42574
参考文献
参考文献
Choudhary C., et al. Science 325:834-840(2009).
Fernandes-Alnemri T., et al. J. Biol. Chem. 269:30761-30764(1994).
Tewari M., et al. Cell 81:801-809(1995).
Pelletier M., et al. Biochem. Biophys. Res. Commun. 316:93-99(2004).
Ota T., et al. Nat. Genet. 36:40-45(2004).

实验结果图

Mouse anti-CASP3 Monoclonal Antibody(1262CT521.280.101)

CASP3 Antibody (Cat. #JP100394) western blot analysis in Jurkat,Ramos cell line lysates (35μg/lane).This demonstrates the CASP3 antibody detected the CASP3 protein (arrow).