Rabbit anti-CD73 Recombinant Monoclonal Antibody(218-52)别名宿主反应种属应用免疫原形式浓度纯化方法类型克隆号储存/保存方法存储溶液背景说明细胞定位UniProt
| 概述 | |
| 别名 |
5′-nucleotidase; 5′-NT; 5′-deoxynucleotidase
|
| 宿主 |
Rabbit
|
| 反应种属 |
Human, Mouse, Rat
|
| 应用 |
WB: 1:1000, IHC-P: 1:500-1:1000
|
| 免疫原 |
Synthetic peptide
|
| 性能 | |
| 形式 |
Liquid
|
| 浓度 |
0.5 mg/ml
|
| 纯化方法 |
Protein A affinity column
|
| 类型 |
Monoclonal Antibody
|
| 克隆号 |
218-52
|
| 储存/保存方法 |
Store at -20℃ for one year.
|
| 存储溶液 |
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
|
| 靶标 | |
| 背景说明 |
CD73, also known as ecto-5′-nucleotidase (ecto-5′-NT, EC 3.1.3.5) is a glycosylphosphatidyl inositol (GPI)-anchored cell surface protein that is encoded by NT5E gene. CD73 is widely expressed on different tissues [PubMed: 9553767, PubMed: 18404475] and cell types including, but not limited to the subsets of T cells and B cells [PubMed: 2550543], endothelial cells [PubMed: 9015312, PubMed: 15358667] and epithelial cells [PubMed: 9169488].
|
| 细胞定位 |
Cell membrane
|
| UniProt |
P21589
|
实验结果图
WB result of CD73 Rabbit mAb Primary antibody: CD73 Rabbit mAb at 1/1000 dilution Lane 1: Raji whole cell lysate 20 µg Lane 2: HT-29 whole cell lysate 20 µg Lane 3: A431 whole cell lysate 20 µg Lane 4: HepG2 whole cell lysate 20 µg Negative control: Raji whole cell lysate Secondary antibody: #JP20040 at 1/10000 dilution Predicted MW: 63 kDa Observed MW: 70 kDa Exposure time: 120s
WB result of CD73 Rabbit mAb Primary antibody: CD73 Rabbit mAb at 1/1000 dilution Lane 1: mouse brain lysate 20 µg Secondary antibody: #JP20040 at 1/10000 dilution Predicted MW: 63 kDa Observed MW: 68 kDa Exposure time: 180s
WB result of CD73 Rabbit mAb Primary antibody: CD73 Rabbit mAb at 1/1000 dilution Lane 1: rat brain lysate 20 µg Secondary antibody: #JP20040 at 1/10000 dilution Predicted MW: 63 kDa Observed MW: 70 kDa Exposure time: 180s
IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD73 antibody was used at 1/500 dilution, Secondary antibody: #JP20025. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-CD73 antibody was used at 1/500 dilution, Secondary antibody: #JP20025. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-CD73 antibody was used at 1/1000 dilution, Secondary antibody: #JP20025. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-CD73 antibody was used at 1/500 dilution, Secondary antibody: #JP20025. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-CD73 antibody was used at 1/500 dilution, Secondary antibody: #JP20025. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat liver. Anti-CD73 antibody was used at 1/1000 dilution, Secondary antibody: #JP20025. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.