Mouse anti-USP11 Monoclonal Antibody(C-term R565)(1220CT620.193.189)描述别名宿主特异性反应种属应用分子量类型克隆号同种型储存/保存方法研究领域背景说明细胞定位UniProt参考文献
| 概述 | |
| 描述 |
Purified Mouse Monoclonal Antibody (Mab)
|
| 别名 |
USP11抗体;Ubiquitin carboxyl-terminal hydrolase 11; Deubiquitinating enzyme 11; Ubiquitin thioesterase 11; Ubiquitin-specific-processing protease 11; USP11; UHX1
|
| 宿主 |
Mouse
|
| 特异性 |
This USP11 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 32-300 amino acids from the C-terminal region of human USP11.
|
| 反应种属 |
Human
|
| 应用 |
FC~~1:25
WB~~1:1000 |
| 分子量 |
Predicted molecular weight: 110kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 性能 | |
| 类型 |
Monoclonal Antibody
|
| 克隆号 |
1220CT620.193.189
|
| 同种型 |
IgG1,κ
|
| 储存/保存方法 |
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
|
| 研究领域 |
Cell Biology
|
| 靶标 | |
| 背景说明 |
Protease that can remove conjugated ubiquitin from target proteins and polyubiquitin chains. Inhibits the degradation of target proteins by the proteasome. Plays a role in the regulation of pathways leading to NF-kappa-B activation. Plays a role in the regulation of DNA repair after double-stranded DNA breaks.
|
| 细胞定位 |
Nucleus. Cytoplasm. Note=Predominantly nuclear. Associates with chromatin
|
| UniProt |
P51784
|
| 参考文献 | |
| 参考文献 |
Ross M.T.,et al.Nature 434:325-337(2005).
Ideguchi H.,et al.Biochem. J. 367:87-95(2002). Swanson D.A.,et al.Hum. Mol. Genet. 5:533-538(1996). Schoenfeld A.R.,et al.Mol. Cell. Biol. 24:7444-7455(2004). Yamaguchi T.,et al.J. Biol. Chem. 282:33943-33948(2007). |
实验结果图
Flow cytometric analysis of Hela cells using USP11 Antibody (C-term R565)(green, Cat#JP100414) compared to an isotype control of mouse IgG1(blue). JP100414 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.
Western blot analysis of lysates from Jurkat, Hela, LNCaP cell line (from left to right) using USP11 Antibody (C-term R565) (Cat. # JP100414). JP100414 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.