Rabbit anti-IKK α/β(pS180/181) Polyclonal Antibody

Rabbit anti-IKK α/β(pS180/181) Polyclonal Antibody描述别名宿主特异性反应种属应用分子量免疫原纯化方法类型储存/保存方法UniProt

概述
描述
Rabbit polyclonal antibody to IKK alpha/beta (pS180/181)
别名
IKK alpha抗体, beta抗体;CHUK; IKKA; TCF16; Inhibitor of nuclear factor kappa-B kinase subunit alpha; I-kappa-B kinase alpha; IKK-A; IKK-alpha; IkBKA; IkappaB kinase; Conserved helix-loop-helix ubiquitous kinase; I-kappa-B kinase 1; IKK1; Nuclear factor NF-kappa-B inhibitor kinase alpha; NFKBIKA; Transcription factor 16; TCF-16; IKBKB; IKKB; Inhibitor of nuclear factor kappa-B kinase subunit beta; I-kappa-B-kinase beta; IKK-B; IKK-beta; IkBKB; I-kappa-B kinase 2; IKK2; Nuclear factor NF-kappa-B inhibitor kinase beta; NFKBIKB
宿主
Rabbit
特异性
Recognizes endogenous levels of IKK alpha/beta (pS180/181) protein.
反应种属
Human, Mouse, Rat, Bovine, Chicken, Zebrafish
应用
WB 1:500 – 1:1000, IHC 1:50 – 1:200
分子量
Detects a band of approximately 85kD(Predicted molecular weight: 84/86kD)
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
免疫原
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human IKK alpha/beta. The exact sequence is proprietary.
性能
纯化方法
The antibody was purified by immunogen affinity chromatography.
类型
Polyclonal Antibody
储存/保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
靶标
UniProt
O15111

实验结果图

Rabbit anti-IKK α/β(pS180/181) Polyclonal Antibody

Western blot analysis of IKK alpha/beta (pS180/181) expression in HEK293T LPS-treated (A), Raw264.7 TNFa-treated (B), PC12 LPS-treated (C) whole cell lysates.

Rabbit anti-IKK α/β(pS180/181) Polyclonal Antibody

Immunohistochemical analysis of IKK alpha/beta (pS180/181) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.