Mouse anti-USP5 Monoclonal Antibody(1340CT704.170.140)描述别名宿主特异性反应种属应用分子量类型克隆号同种型储存/保存方法研究领域背景说明UniProt参考文献
| 概述 | |
| 描述 |
Purified Mouse Monoclonal Antibody (Mab)
|
| 别名 |
USP5抗体;Ubiquitin carboxyl-terminal hydrolase 5; Deubiquitinating enzyme 5; Isopeptidase T; Ubiquitin thioesterase 5; Ubiquitin-specific-processing protease 5; USP5; ISOT
|
| 宿主 |
Mouse
|
| 特异性 |
This USP5 antibody is generated from a mouse immunized with a recombination protein from the human region of human USP5.
|
| 反应种属 |
Human, Mouse, Rat
|
| 应用 |
WB~~1:2000
|
| 分子量 |
Predicted molecular weight: 96kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 性能 | |
| 类型 |
Monoclonal Antibody
|
| 克隆号 |
1340CT704.170.140
|
| 同种型 |
IgG1,κ
|
| 储存/保存方法 |
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
|
| 研究领域 |
Cell Biology
|
| 靶标 | |
| 背景说明 |
Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored ‘Lys-48’-linked polyubiquitin disassembly. Binds linear and ‘Lys- 63’-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.
|
| UniProt |
P45974
|
| 参考文献 | |
| 参考文献 |
Falquet L.,et al.FEBS Lett. 376:233-237(1995).
Ansari-Lari M.A.,et al.Genome Res. 6:314-326(1996). Ansari-Lari M.A.,et al.Genome Res. 7:268-280(1997). Tashayev V.L.,et al.Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases. Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases. |
实验结果图
Western blot analysis of lysates from U251, PC-3 cell line, mouse brain, rat brain and liver tissue lysates (from left to right), using USP5 Antibody(Cat. #JP100451). JP100451 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.