Rabbit anti-OCT3/4 Recombinant Monoclonal Antibody(165-76)别名宿主反应种属应用免疫原形式浓度纯化方法类型克隆号储存/保存方法存储溶液背景说明细胞定位UniProt
| 概述 | |
| 别名 |
POU5F1; OTF-3
|
| 宿主 |
Rabbit
|
| 反应种属 |
Human
|
| 应用 |
WB: 1:500, IP: 1:25, IHC-P: 1:1000
|
| 免疫原 |
Synthetic peptide
|
| 性能 | |
| 形式 |
Liquid
|
| 浓度 |
0.25 mg/ml
|
| 纯化方法 |
Protein A affinity column
|
| 类型 |
Monoclonal Antibody
|
| 克隆号 |
165-76
|
| 储存/保存方法 |
Store at -20℃ for one year.
|
| 存储溶液 |
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
|
| 靶标 | |
| 背景说明 |
Oct-4, a transcription factor also known as Oct-3, Oct-3/4, Otf3 or NF-A3, is encoded by the Pou5f1 gene (located on chromosome 6 in human and 17 in mouse) and belongs to the POU (Pit, Oct, Unc) family of DNA binding-proteins. These proteins regulate the expression of target genes by binding to the octamer motif ATGCAAAT within their promoter or enhancer regions. Oct4, whose expression is associated with pluripotent properties of stem cells, is an essential factor controlling early stages of mammalian embryogenesis.
|
| 细胞定位 |
Nucleus
|
| UniProt |
Q01860
|
实验结果图
WB result of OCT3/4 Rabbit mAb Primary antibody: OCT3/4 Rabbit mAb at 1/500 dilution Lane 1: HeLa whole cell lysate 20 µg Lane 2: NCCIT whole cell lysate 20 µg Negative control: HeLa whole cell lysate Secondary antibody: #JP20040 at 1/10000 dilution Predicted MW: 45 kDa Observed MW: 40 kDa Exposure time: 60s
IHC shows positive staining in paraffin-embedded human seminoma. Anti-OCT3/4 antibody was used at 1/1000 dilution, Secondary antibody: #JP20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human hepatocellular carcinoma. Anti-OCT3/4 antibody was used at 1/1000 dilution, Secondary antibody: #JP20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human cervical carcinoma. Anti-OCT3/4 antibody was used at 1/1000 dilution, Secondary antibody: #JP20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-OCT3/4 antibody was used at 1/1000 dilution, Secondary antibody: #JP20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human testis. Anti-OCT3/4 antibody was used at 1/1000 dilution, Secondary antibody: #JP20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
OCT3/4 Rabbit mAb at 1/25 dilution (1µg) immunoprecipitating OCT3/4 in 0.4mg NCCIT whole cell lysate. Western blot was performed on the immunoprecipitate using OCT3/4 Rabbit mAb at 1/1000 dilution. Secondary antibody (HRP) for IP was used at 1/400 dilution. Lane 1 : NCCIT whole cell lysate 10µg(input) Lane 2 : OCT3/4 Rabbit mAb IP in NCCIT whole cell lysate Lane 3 : Rabbit monoclonal IgG IP in NCCIT whole cell lysate Predicted MW: 45 kDa Observed MW: 40 kDa Exposure time: 20s