Mouse anti-PPP2R1B Monoclonal Antibody(1496CT356.164.25.226)描述别名宿主特异性反应种属应用分子量类型克隆号同种型储存/保存方法研究领域背景说明UniProt参考文献
| 概述 | |
| 描述 |
Purified Mouse Monoclonal Antibody (Mab)
|
| 别名 |
PPP2R1B抗体;Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform; PP2A subunit A isoform PR65-beta; PP2A subunit A isoform R1-beta; PPP2R1B
|
| 宿主 |
Mouse
|
| 特异性 |
This PPP2R1B antibody is generated from a mouse immunized with a recombinant protein of human PPP2R1B.
|
| 反应种属 |
Human, Mouse, Rat
|
| 应用 |
IF~~1:25
IHC-P~~1:25 FC~~1:25 WB~~1:4000 |
| 分子量 |
Predicted molecular weight: 66kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 性能 | |
| 类型 |
Monoclonal Antibody
|
| 克隆号 |
1496CT356.164.25.226
|
| 同种型 |
IgG1,Κ
|
| 储存/保存方法 |
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
|
| 研究领域 |
Cell Biology;Signal Transduction
|
| 靶标 | |
| 背景说明 |
The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit.
|
| UniProt |
P30154
|
| 参考文献 | |
| 参考文献 |
Baysal B.E.,et al.Gene 217:107-116(1998).
Wang S.S.,et al.Science 282:284-287(1998). Baysal B.E.,et al.Eur. J. Hum. Genet. 9:121-129(2001). Ota T.,et al.Nat. Genet. 36:40-45(2004). Taylor T.D.,et al.Nature 440:497-500(2006). |
实验结果图
Overlay histogram showing Jurkat cells stained with JP100500 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (JP100500, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(NA168821)) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 (1μg/1×10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
All lanes : Anti-PPP2R1B Antibody at 1:4000 dilution Lane 1: Jurkat whole cell lysates Lane 2: human brain lysates Lane 3: mouse brain lysates Lane 4: mouse lung lysates Lane 5: rat brain lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 66 kDa Blocking/Dilution buffer: 5% NFDM/TBST.