Purified Mouse Monoclonal Antibody (Mab)

RPTOR抗体;Regulatory-associated protein of mTOR; Raptor; p150 target of rapamycin (TOR)-scaffold protein; RPTOR; KIAA1303; RAPTOR

Mouse

This RPTOR antibody is generated from a mouse immunized with recombinant protein.

Human, Mouse, Rat

IHC-P~~1:25
WB~~1:500-1:1000

Predicted molecular weight: 149kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Monoclonal Antibody

1411CT316.2.151.34

IgG1,k

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cell Biology;Signal Transduction

Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1- mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at ‘Thr-389’, which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Involved in ciliogenesis.

Cytoplasm. Lysosome. Cytoplasmic granule. Note=Targeting to lysosomes depends on amino acid availability. In arsenite-stressed cells, accumulates in stress granules when associated with SPAG5 and association with lysosomes is drastically decreased

Q8N122

Kim D.-H.,et al.Cell 110:163-175(2002).
Hara K.,et al.Cell 110:177-189(2002).
Zody M.C.,et al.Nature 440:1045-1049(2006).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Nagase T.,et al.DNA Res. 7:65-73(2000).