Recombinant Human Fibroblast Growth Factor-basic (147 a.a)描述来源序列纯度储存/保存方法技术指标产品用途别名外观可溶性/溶解性
| 产品描述 | |
| 描述 |
FGF-basic is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different isoforms have been described. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.
Fibroblast Growth Factor-2 Human Recombinant (FGF-2) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 147 amino acids and having a molecular mass of 16.5kDa.
The FGF2 is purified by proprietary chromatographic techniques.
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| 来源 |
Escherichia Coli
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| 序列 |
MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS.
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| 纯度 |
95%
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| 储存/保存方法 |
Lyophilized basic-FGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGFb should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
| 技术指标 |
BIOLOGICAL ACTIVITY：
The ED50, Calculated by the dose- dependent proliferation of mouse BALB/c 3T3 cells is 22.5pg/ml corresponding to a specific activity of 4.4×107 units/mg. |
| 产品用途 |
products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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| 基本信息 | |
| 别名 |
重组人碱性成纤维细胞生长因子（147 a.a）；FGF 2 （147 a.a.）
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| 外观 |
Sterile Filtered White lyophilized (freeze-dried) powder.
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| 可溶性/溶解性 |
It is recommended to reconstitute the lyophilized FGF-B in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
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