Rabbit anti-CD146 Polyclonal Antibody别名宿主特异性反应种属预测反应种属应用分子量免疫原浓度纯化方法类型同种型储存/保存方法存储溶液研究领域使用方法背景说明细胞定位UniProt
| 概述 | |
| 别名 |
CD146抗体;A32 antigen; CD 146; CD146; CD146 antigen; Cell surface glycoprotein MUC18; Cell surface glycoprotein P1H12; MCAM; Melanoma adhesion molecule; Melanoma associated antigen A32; Melanoma associated antigen MUC18; Melanoma associated glycoprotein MUC18; Melanoma cell adhesion molecule; MelCAM; MUC 18; MUC18; S endo 1; S endo 1 endothelial associated antigen; Sendo 1 endothelial associated antigen; Sendo1; MUC18_HUMAN
|
| 宿主 |
Rabbit
|
| 特异性 |
CD146
|
| 反应种属 |
Human, Rat
|
| 预测反应种属 |
Mouse;Dog;Cow;Horse;
|
| 应用 |
WB=1:500-2000
|
| 分子量 |
Predicted molecular weight: 71kDa
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 免疫原 |
KLH conjugated synthetic peptide derived from human CD146 (203-240aa):201-300/646
|
| 性能 | |
| 浓度 |
1mg/ml
|
| 纯化方法 |
affinity purified by Protein A
|
| 类型 |
Polyclonal Antibody
|
| 同种型 |
IgG
|
| 储存/保存方法 |
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
|
| 存储溶液 |
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
|
| 研究领域 |
肿瘤 免疫学 细胞粘附分子 细胞表面分子
|
| 使用方法 |
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC= IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 靶标 | |
| 背景说明 |
MCAM (MUC18 antigen, CD146), a member of the immuoglobulin superfamily, is an intrinsic membrane glycoprotein of 110-120 kDa found on the surface of endothelial cells, bone marrow fibroblasts and various melanomas. MCAM (Melanoma adhesion molecule) has been used as a marker of tumor progression in human melanoma because expression in those tumors correlates strongly with poor prognosis and the development of metastic disease. In addition, a number of human T, B and myeloid leukemic cell lines seem to express MCAM. The close structural relationship with N-CAM and related molecules suggests that MCAM may be also a developmentally regulated cell adhesion.
|
| 细胞定位 |
细胞膜
|
| UniProt |
P43121
|