C25-140CAS号： 1358099-18-9分子式： C26H31N7O分子量： 457.57描述纯度储存/保存方法可溶性/溶解性靶点In vitro(体外研究)In vivo(体内研究)
| 产品描述 | |
| 描述 |
C25-140, a first-in-class TRAF6-Ubc13 inhibitor, directly binds to TRAF6, thereby blocks the interaction of TRAF6 with Ubc13 and as a consequence lowers TRAF6 activity and combats autoimmunity.
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| 纯度 |
98%
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| 储存/保存方法 |
Store at -20℃ for one year（Powder）；Store at 2-4℃ for two weeks；Store at -20℃ for six months after dissolution.
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| 基本信息 | |
| 可溶性/溶解性 |
DMSO : 41.67 mg/mL (91.07 mM)
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| 生物活性 | |
| 靶点 |
TRAF6-Ubc13
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| In vitro(体外研究) |
C25-140 dose-dependently impedes TRAF6-Ubc13 interaction.C25-140 (10-30 μM; 2 hours) effectively reduces TRAF6-mediated ubiquitin chain formation.C25-140 affects TNFα-induced phosphorylation of IκBα as well as NF-κB-induced target gene expression.C25-140 efficiently inhibits IL-1β- and TNFα-mediated receptor signaling in the context of cytokine activation.Western Blot AnalysisCell Line:TRAF6WTConcentration:10 μM, 20 μM, 30 μMIncubation Time:2 hoursResult:Effectively reduced TRAF6-mediated ubiquitin chain formation.
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| In vivo(体内研究) |
C25-140 dose-dependently impedes TRAF6-Ubc13 interaction.C25-140 (10-30 μM; 2 hours) effectively reduces TRAF6-mediated ubiquitin chain formation.C25-140 affects TNFα-induced phosphorylation of IκBα as well as NF-κB-induced target gene expression.C25-140 efficiently inhibits IL-1β- and TNFα-mediated receptor signaling in the context of cytokine activation.Western Blot AnalysisCell Line:TRAF6WTConcentration:10 μM, 20 μM, 30 μMIncubation Time:2 hoursResult:Effectively reduced TRAF6-mediated ubiquitin chain formation.
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分子结构图
