Mouse anti-GATA3 Monoclonal Antibody(Center)(1259CT707.136.102)描述别名宿主特异性反应种属应用分子量类型克隆号同种型储存/保存方法研究领域背景说明细胞定位UniProt参考文献
| 概述 | |
| 描述 |
Purified Mouse Monoclonal Antibody (Mab)
|
| 别名 |
GATA3抗体;Trans-acting T-cell-specific transcription factor GATA-3; GATA-binding factor 3; GATA3
|
| 宿主 |
Mouse
|
| 特异性 |
This GATA3 antibody is generated from a mouse immunized with a recombinant protein from human GATA3.
|
| 反应种属 |
Human
|
| 应用 |
IF~~1:25
FC~~1:25 WB~~1:1000 |
| 分子量 |
Predicted molecular weight: 48kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 性能 | |
| 类型 |
Monoclonal Antibody
|
| 克隆号 |
1259CT707.136.102
|
| 同种型 |
IgG2b,κ
|
| 储存/保存方法 |
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
|
| 研究领域 |
Developmental Biology;Immunology;Signal Transduction
|
| 靶标 | |
| 背景说明 |
Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5′-AGATAG-3′. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses.
|
| 细胞定位 |
Nucleus.
|
| UniProt |
P23771
|
| 参考文献 | |
| 参考文献 |
Joulin V.,et al.EMBO J. 10:1809-1816(1991).
Ho I.-C.,et al.EMBO J. 10:1187-1192(1991). Ko L.J.,et al.Mol. Cell. Biol. 11:2778-2784(1991). Marine J.,et al.Proc. Natl. Acad. Sci. U.S.A. 88:7284-7288(1991). Deloukas P.,et al.Nature 429:375-381(2004). |
实验结果图
Fluorescent image of MCF-7 cells stained with GATA3 Antibody (Center)(Cat#JP100417). JP100417 was diluted at 1:25 dilution. An Alexa Fluor® 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
Flow cytometric analysis of MCF-7 cells using GATA3 Antibody (Center)(green, Cat#JP100417) compared to an isotype control of mouse IgG2b(blue). JP100417 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.
Western blot analysis of lysates from CEM, HL-60, SH-SY5Y cell lines (from left to right) using GATA3 Antibody (Center) (Cat. # JP100417). JP100417 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.