Purified Mouse Monoclonal Antibody (Mab)

ATG5抗体;Autophagy protein 5; APG5-like; Apoptosis-specific protein; ATG5; APG5L; ASP

Mouse

This ATG5 antibody is generated from a mouse immunized with a recombination protein from the human region of human ATG5.

Human, Mouse, Rat

Bovine, Pig

WB~~1:1000

Predicted molecular weight: 32kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Monoclonal Antibody

1358CT289.125.123

IgG1,κ

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cancer;Cardiovascular;Cell Biology;Metabolism

Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. The ATG12- ATG5 conjugate also negatively regulates the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS. Also plays a role in translation or delivery of incoming viral RNA to the translation apparatus. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway.

Cytoplasm. Preautophagosomal structure membrane; Peripheral membrane protein. Note=Colocalizes with nonmuscle actin. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (By similarity). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme

Q9H1Y0

Hammond E.M.,et al.FEBS Lett. 425:391-395(1998).
Chen Y.,et al.Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases.
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Mungall A.J.,et al.Nature 425:805-811(2003).
Grand R.J.A.,et al.Exp. Cell Res. 218:439-451(1995).