Rabbit anti-PCNA Recombinant Monoclonal Antibody(297-116)

Rabbit anti-PCNA Recombinant Monoclonal Antibody(297-116)描述别名宿主特异性反应种属应用免疫原形式浓度纯化方法类型克隆号储存/保存方法存储溶液背景说明翻译后修饰细胞定位UniProt

概述
描述	Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication (1). Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix (2). Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA (reviewed in 3). PCNA protein expression is a well-accepted marker of proliferation and PCNA (PC10) Mouse mAb has been used to assess PCNA levels in hundreds of scientific studies.
别名	PCNA抗体;Proliferating cell nuclear antigen; Cyclin
宿主	Rabbit
特异性	PCNA Antibody detects endogenous levels of total PCNA.
反应种属	Human, Mouse, Rat
应用	WB: 1: 1000, IP: 1: 50, IHC-P: 1: 2000, ICC: 1: 500, FC(Intra): 1:50
免疫原	Synthetic peptide
性能
形式	Liquid
浓度	0.5 mg/mL
纯化方法	Protein A affinity column
类型	Monoclonal Antibody
克隆号	297-116
储存/保存方法	Store at -20℃ for one year.
存储溶液	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
靶标
背景说明	Proliferating cell nuclear antigen (PCNA) is a type of DNA clamp that acts as a DNA polymerase in eukaryotic cells δ. The processing factor of is crucial for replication. PCNA is a homologous trimer that achieves its processability by surrounding DNA, where it serves as a scaffold for recruiting proteins involved in DNA replication, DNA repair, chromatin remodeling, and epigenetics. It is expressed in most tumors as a proliferative antigen.
翻译后修饰	Phosphorylated. Phosphorylation at Tyr-211 by EGFR stabilizes chromatin-associated PCNA.Acetylated by CREBBP and p300/EP300; preferentially acetylated by CREBBP on Lys-80, Lys-13 and Lys-14 and on Lys-77 by p300/EP300 upon loading on chromatin in response to UV irradiation (PubMed:24939902, PubMed:19419956). Lysine acetylation disrupts association with chromatin, hence promoting PCNA ubiquitination and proteasomal degradation in response to UV damage in a CREBBP- and EP300-dependent manner (PubMed:24939902). Acetylation disrupts interaction with NUDT15 and promotes degradation (PubMed:19419956).Ubiquitinated (PubMed:24939902, PubMed:20227374). Following DNA damage, can be either monoubiquitinated to stimulate direct bypass of DNA lesions by specialized DNA polymerases or polyubiquitinated to promote recombination-dependent DNA synthesis across DNA lesions by template switching mechanisms. Following induction of replication stress, monoubiquitinated by the UBE2B-RAD18 complex on Lys-164, leading to recruit translesion (TLS) polymerases, which are able to synthesize across DNA lesions in a potentially error-prone manner. An error-free pathway also exists and requires non-canonical polyubiquitination on Lys-164 through ‘Lys-63’ linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH. This error-free pathway, also known as template switching, employs recombination mechanisms to synthesize across the lesion, using as a template the undamaged, newly synthesized strand of the sister chromatid. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis. Sumoylated during S phase.Methylated on glutamate residues by ARMT1/C6orf211.
细胞定位	Nucleus
UniProt	P12004

实验结果图