Rabbit anti-Myosin-11 Recombinant Monoclonal Antibody(188-112)别名宿主反应种属应用免疫原形式浓度纯化方法类型克隆号储存/保存方法存储溶液背景说明细胞定位UniProt
| 概述 | |
| 别名 |
SMMHC; smooth muscle myosin heavy chain; HSM-V; SMMS-1; SM-MYOSIN-H; Myosin heavy chain 11; MYH11
|
| 宿主 |
Rabbit
|
| 反应种属 |
Human, Mouse, Rat
|
| 应用 |
WB: 1:1000-1:25000, IHC-P: 1:1000, IF: 1:125
|
| 免疫原 |
Synthetic peptide
|
| 性能 | |
| 形式 |
Liquid
|
| 浓度 |
0.25 mg/ml
|
| 纯化方法 |
Protein A affinity column
|
| 类型 |
Monoclonal Antibody
|
| 克隆号 |
188-112
|
| 储存/保存方法 |
Store at -20℃ for one year.
|
| 存储溶液 |
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
|
| 靶标 | |
| 背景说明 |
SMMHC is a structural component of the smooth muscle myosin molecule and is a specific marker of “terminal” smooth muscle differentiation [PMID: 10721417, PMID: 3882826]. SMMHC is composed of at least two isoforms: SM1 (204 kDa) and SM2 (200 kDa), both of which are encoded by a single gene [PMID: 9702855, PMID: 10998642]. The SM1 isoform is expressed in the MEC of normal mammary glands, fibrocystic diseases, and in myoepithelial derived tumours of the breast [PMID: 11493962, PMID: 9702855]. Furthermore, studies have documented that antibodies to SMMHC and calponin, both markers of terminal smooth muscle differentiation, are more specific for breast MEC than are other more commonly used antibodies, such as those that recognise SMA [PMID: 11493962].
|
| 细胞定位 |
Melanosome
|
| UniProt |
P35749
|
实验结果图
WB result of Myosin-11 Rabbit mAb Primary antibody: Myosin-11 Rabbit mAb at 1/25000 dilution Lane 1: mouse skeletal muscle lysate 5ug Lane 2: mouse bladder lysate 5ug Negative control: mouse skeletal muscle lysate Secondary antibody: #JP20040 at 1/10000 dilution Predicted MW: 227kDa Observed MW: 250kDa Exposure time: 30s
WB result of Myosin-11 Rabbit mAb Primary antibody: Myosin-11 Rabbit mAb at 1/1000 dilution Lane 1: mouse skeletal muscle lysate 20ug Lane 2: mouse prostate lysate 20ug Negative control: mouse skeletal muscle lysate Secondary antibody: #JP20040 at 1/10000 dilution Predicted MW: 227kDa Observed MW: 250kDa Exposure time: 60s
WB result of Myosin-11 Rabbit mAb Primary antibody: Myosin-11 Rabbit mAb at 1/25000 dilution Lane 1: rat skeletal muscle lysate 5ug Lane 2: rat bladder lysate 5ug Negative control: rat skeletal muscle lysate Secondary antibody: #JP20040 at 1/10000 dilution Predicted MW: 227kDa Observed MW: 250kDa Exposure time: 30s
IHC shows positive staining in paraffin-embedded human breast. Anti-Myosin-11 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse stomach. Anti-Myosin-11 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach. Anti-Myosin-11 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-Myosin-11 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-Myosin-11 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-Myosin-11 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Myosin-11 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human leiomyosarcoma. Anti-Myosin-11 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IF shows positive staining in paraffin-embedded human colon. Anti-Myosin-11 antibody was used at 1/125 dilution (Green). Secondary antibody: #JP20025 at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded mouse colon. Anti-Myosin-11 antibody was used at 1/125 dilution (Green). Secondary antibody: #JP20025 at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.