Rabbit anti-CytokeRatin 19 Recombiant Monoclonal Antibody(107-19)别名宿主反应种属应用免疫原形式浓度纯化方法类型克隆号储存/保存方法存储溶液背景说明细胞定位UniProt
| 概述 | |
| 别名 |
KRT19; CK-19
|
| 宿主 |
Rabbit
|
| 反应种属 |
Human, Mouse, Rat
|
| 应用 |
WB: 1:500, IHC:1:1000, FC: 1:500
|
| 免疫原 |
Synthetic peptide
|
| 性能 | |
| 形式 |
liquid
|
| 浓度 |
0.25mg/ml
|
| 纯化方法 |
Protein A affinity column
|
| 类型 |
Monoclonal antibody
|
| 克隆号 |
107-19
|
| 储存/保存方法 |
Store at -20℃ for one year.
|
| 存储溶液 |
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
|
| 靶标 | |
| 背景说明 |
Cytokeratin 19 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Keratin 19 is a type I keratin. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically found in the periderm, the transiently superficial layer that envelops the developing epidermis. KRT19 is also known as Cyfra 21-1.Due to its high sensitivity, KRT19 is the most used marker for the RT-PCR-mediated detection of tumor cells disseminated in lymph nodes, peripheral blood, and bone marrow of breast cancer patients.
|
| 细胞定位 |
Cell membrane
|
| UniProt |
P08727
|
实验结果图
WB result of Cytokeratin 19 Rabbit mAb Primary antibody: Cytokeratin 19 Rabbit mAb at 1/500 dilution Lane 1: Hela whole cell lysate 20 µg Lane 2: MCF7 whole cell lysate 20 µg Lane 3: HT-29 whole cell lysate 20 µg Lane 4: HepG2 whole cell lysate 20 µgSecondary antibody: #JP20040 at 1/10000 dilution Predicted MW: 41 kDa Observed MW: 39 kDa Exposure time: 20s
WB result of Cytokeratin 19 Rabbit mAb Primary antibody: Cytokeratin 19 Rabbit mAb at 1/500 dilution Lane 1: mouse kidney lysate 20 µg Secondary antibody: #JP20040 at 1/10000 dilution Predicted MW: 41 kDa Observed MW: 39 kDa Exposure time: 120 s
WB result of Cytokeratin 19 Rabbit mAb Primary antibody: Cytokeratin 19 Rabbit mAb at 1/500 dilution Lane 1: rat kidney whole cell lysate 20 µg Secondary antibody: #JP20040 at 1/10000 dilution Predicted MW: 41 kDa Observed MW: 39 kDa Exposure time: 120 s
IHC shows positive staining in paraffin-embedded human colon. Anti-Cytokeratin19 antibody was used at 1/1000 dilution, Secondary antibody: #JP20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human placenta. Anti-Cytokeratin19 antibody was used at 1/1000 dilution, Secondary antibody: #JP20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-Cytokeratin19 antibody was used at 1/1000 dilution, Secondary antibody: #JP20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervix cancer. Anti-Cytokeratin19 antibody was used at 1/1000 dilution, Secondary antibody: #JP20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse stomach. Anti-Cytokeratin19 antibody was used at 1/1000 dilution, Secondary antibody: #JP20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-Cytokeratin19 antibody was used at 1/1000 dilution, Secondary antibody: #JP20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Flow cytometric analysis of MCF7 cells labelling Cytokeratin 19 antibody at 1/500 dilution/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Secondary antibody: #JP20040 at 1/1000 dilution was used as the secondary antibody.