Mouse Monoclonal Antibody (Mab)

H2AFX抗体;Histone H2AX; H2a/x; H2AFX; H2AX

Mouse

This H2AFX antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human H2AFX.

Human

Z, M

IHC-P~~1:25
WB~~1:1000

Predicted molecular weight: 15kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Monoclonal Antibody

980CT1.5.5

IgG1

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Crown Antibodies

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C- terminal phosphorylation.

Nucleus. Chromosome.

P16104

Stewart G.S., et al. Nature 421:961-966(2003).
Park E.-J., et al. Nucleic Acids Res. 31:6819-6827(2003).
Stiff T., et al. Cancer Res. 64:2390-2396(2004).
Lukas C., et al. EMBO J. 23:2674-2683(2004).
Kurz E.U., et al. J. Biol. Chem. 279:53272-53281(2004).