Mouse anti-CDX1 Monoclonal Antibody(C-term)(937CT11.6.2)描述别名宿主特异性反应种属应用分子量类型克隆号同种型储存/保存方法存储溶液研究领域背景说明细胞定位UniProt参考文献
| 概述 | |
| 描述 |
Mouse Monoclonal Antibody (Mab)
|
| 别名 |
CDX1抗体;Homeobox protein CDX-1; Caudal-type homeobox protein 1; CDX1
|
| 宿主 |
Mouse
|
| 特异性 |
Purified His-tagged CDX1 protein was used to produced this monoclonal antibody.
|
| 反应种属 |
Human, Mouse
|
| 应用 |
WB~~1:1000
|
| 分子量 |
Predicted molecular weight: 28kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 性能 | |
| 类型 |
Monoclonal Antibody
|
| 克隆号 |
937CT11.6.2
|
| 同种型 |
IgG1
|
| 储存/保存方法 |
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
|
| 存储溶液 |
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
|
| 研究领域 |
Crown Antibodies
|
| 靶标 | |
| 背景说明 |
Could play a role in the terminal differentiation of the intestine.
|
| 细胞定位 |
Nucleus.
|
| UniProt |
P47902
|
| 参考文献 | |
| 参考文献 |
Bonner C.A., et al. Genomics 28:206-211(1995).
Mallo G.V., et al. Int. J. Cancer 74:35-44(1997). Malakooti J., et al. Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases. |
实验结果图
All lanes : Anti-CDX1 (C-term) at 1:1000-1:2000 dilution Lane 1: mouse liver lysate Lane 2: mouse testis lysate Lane 3: human testis lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 28 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes : Anti-CDX1 Antibody (C-term) at 1:1000-1:2000 dilution Lane 1: mouse liver lysate Lane 2: mouse testis lysate Lane 3: WiDr whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 28 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
CDX1 Antibody (C-term) (Cat. #JP100336) western blot analysis in WiDr,Hela cell line and mouse liver tissue lysates (35μg/lane).This demonstrates the CDX1 antibody detected the CDX1 protein (arrow).