Mouse anti-PIN1 Monoclonal Antibody(855CT1.7.5)描述别名宿主特异性反应种属应用分子量类型克隆号同种型储存/保存方法存储溶液研究领域背景说明细胞定位UniProt参考文献
| 概述 | |
| 描述 |
Mouse Monoclonal Antibody (Mab)
|
| 别名 |
PIN1抗体;Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1; Peptidyl-prolyl cis-trans isomerase Pin1; PPIase Pin1; Rotamase Pin1; PIN1
|
| 宿主 |
Mouse
|
| 特异性 |
Purified His-tagged PIN1 protein was used to produced this monoclonal antibody.
|
| 反应种属 |
Human, Mouse, Rat, African Green Monkey
|
| 应用 |
IHC-P~~1:25
FC~~1:25 WB~~1:1000 |
| 分子量 |
Predicted molecular weight: 18kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 性能 | |
| 类型 |
Monoclonal Antibody
|
| 克隆号 |
855CT1.7.5
|
| 同种型 |
IgG1
|
| 储存/保存方法 |
Maintain refrigerated at 2-8°C for up to 2 weeks. For long time storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
|
| 存储溶液 |
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
|
| 研究领域 |
Cell Biology;Neuroscience
|
| 靶标 | |
| 背景说明 |
Essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Catalyzes pSer/Thr-Pro cis/trans isomerizations. Down-regulates kinase activity of BTK. Can transactivate multiple oncogenes and induce centrosome amplification, chromosome instability and cell transformation. Required for the efficient dephosphorylation and recycling of RAF1 after mitogen activation. Binds and targets PML and BCL6 for degradation in a phosphorylation-dependent manner.
|
| 细胞定位 |
Nucleus. Nucleus speckle. Cytoplasm. Note=Colocalizes with NEK6 in the nucleus. Mainly localized in the nucleus but phosphorylation at Ser-71 by DAPK1 results in inhibition of its nuclear localization
|
| UniProt |
Q13526
|
| 参考文献 | |
| 参考文献 |
Ebert L., et al. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
Lu K.P., et al. Nature 380:544-547(1996). Kalnine N., et al. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases. Ota T., et al. Nat. Genet. 36:40-45(2004). Mural R.J., et al. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases. |
实验结果图
Immunohistochemical analysis of paraffin-embedded H. breast section using PIN1 Antibody(Cat#JP100362). JP100362 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
PIN1 Antibody (Cat. #JP100362) western blot analysis in Hela,293,mouse NIH/3T3,PC-12,COS-7 cell line and mouse brain,rat brain tissue lysates (35μg/lane).This demonstrates the PIN1 antibody detected the PIN1 protein (arrow).