Rabbit anti-RNF43 Polyclonal Antibody(C-term)描述别名宿主特异性反应种属应用分子量类型同种型储存/保存方法存储溶液研究领域背景说明细胞定位UniProt参考文献
| 概述 | |
| 描述 |
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
|
| 别名 |
RNF43抗体;E3 ubiquitin-protein ligase RNF43; 632-; RING finger protein 43; RNF43
|
| 宿主 |
Rabbit
|
| 特异性 |
This RNF43 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 543-572 amino acids from the C-terminal region of human RNF43.
|
| 反应种属 |
Human
|
| 应用 |
WB~~1:2000
|
| 分子量 |
Predicted molecular weight: 86kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 性能 | |
| 类型 |
Polyclonal Antibody
|
| 同种型 |
Rabbit Ig
|
| 储存/保存方法 |
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
|
| 存储溶液 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
|
| 研究领域 |
Cancer;Cell Biology
|
| 靶标 | |
| 背景说明 |
E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination, endocytosis and subsequent degradation of Wnt receptor complex components Frizzled. Acts on both canonical and non-canonical Wnt signaling pathway. Acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, thereby resticting the size of the intestinal stem cell zone.
|
| 细胞定位 |
Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Nucleus envelope. Note=According to a report, may be secreted.
|
| UniProt |
Q68DV7
|
| 参考文献 | |
| 参考文献 |
Low, S.K., et al. PLoS ONE 5 (7), E11824 (2010) :
Sugiura, T., et al. Exp. Cell Res. 314(7):1519-1528(2008) Uchida, N., et al. Clin. Cancer Res. 10(24):8577-8586(2004) Yagyu, R., et al. Int. J. Oncol. 25(5):1343-1348(2004) |
实验结果图
Anti-RNF43 Antibody (C-term) at 1:2000 dilution + A549 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 86 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
RNF43 Antibody (C-term) (Cat. #JP101800) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the RNF43 antibody detected the RNF43 protein (arrow).
RNF43 Antibody (C-term) (Cat. #JP101800)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RNF43 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.