Purified Mouse Monoclonal Antibody (Mab)

FZR抗体;Fizzy-related protein homolog; Fzr; CDC20-like protein 1; Cdh1/Hct1 homolog; hCDH1; FZR1; CDH1; FYR; FZR; KIAA1242

Mouse

This FZR antibody is generated from a mouse immunized with a recombinant protein between1-496 amino acids from human FZR.

Human

WB~~1:2000

Predicted molecular weight: 55kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Monoclonal Antibody

1621CT637.76.67

IgG1,k

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cell Biology

Key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis.

Isoform 2: Nucleus.

Q9UM11

Kramer E.R.,et al.Curr. Biol. 8:1207-1210(1998).
Kotani S.,et al.Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases.
Sudo T.,et al.Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases.
Zhou Y.,et al.Biochem. J. 374:349-358(2003).
Nagase T.,et al.DNA Res. 6:337-345(1999).