Human IFN-gammaSEKH-0046
产品名称：Human IFN-gamma
产品型号：SEKH-0046
产品特点：Human IFN-gamma金畔生物ELISA试剂盒的优势：1，包被的酶标板单板可拆（能拆分成12个8孔的酶标条）2，提供免费代测代检服务，代出实验数据3，发文章高奖励4，磁铁可吸附式包装盒，包装精美耐用
SEKH-0046Human IFN-gamma的详细资料：
Human IFN-gamma
| 中文名称 | 人干扰素γ检测试剂盒 |
|---|---|
| 英文名称 | Human IFN-γ ELISA KIT |
| Reactivity | Human |
| Sample type | Serum, plasma, Cell culture supernatant |
| Sensitivity | 4 pg/ml |
| Detection Target | IFN-γ |
| Applications | Sandwich ELISA |
Human IFN-gamma
PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IFN-γ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFN-γ present is captured by the coated antibody after incubation. Following extensive washing, a biotin-conjugate antibody specific for IFN-γ is added to detect the captured IFN-γ protein in sample. For signal development, horseradish peroxidase (HRP)-conjugated Streptavidin is added, followed by Tetramethyl-benzidine (TMB) reagent. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm