细胞培养基 DMEM（H）（含双抗）12100-500
产品名称：细胞培养基 DMEM（H）（含双抗）
产品型号：12100-500
产品特点：细胞培养基 DMEM（H）（含双抗）产品含4.5g/L 葡萄糖 ，含谷氨酰胺，不含丙酮酸钠。质量稳定可靠
12100-500细胞培养基 DMEM（H）（含双抗）的详细资料：
细胞培养基 DMEM（H）（含双抗）
DMEM培养基的配制方法：
1 制备新鲜三蒸水或Millipore超纯水。
2 称取所需量的干粉培养基，加入约终体积一半的三蒸水中;若配制一个包装的培养液，在将整个包装的干粉倒入三蒸水后，需用水洗包装袋内面2次，倒入培养液中，以保证所有干粉都溶解成培养液。磁力搅拌或人工搅拌使之*溶解。
3 根据包装袋上的要求补加所需量的碳酸氢钠;根据实验需要，添HEPES(5-20mmol/L)、谷氨酰胺和其他特殊物质。
4 加水定容到终体积。
5 必要时用1 mol/L 盐酸和1 mol/L 氢氧化钠调节pH。
6 用无菌0.22um滤膜过滤除菌，分装于无菌血清瓶中，4℃冰箱保存。
配制好的培养液用前加入100U/mL青霉素和100U/mL链霉素，并根据需要加入血清(5%-20%)。
DMEM培养基的特点：
（1）氨基酸含量为依格尔培养基的2倍，且含有非必需氨基酸，如甘氨酸等；
（2）维生素含量为依格尔培养基的4倍；
（3）含有糖酵解途径中的重要物质——丙酮酸；
（4）含有微量的铁离子。
DMEM培养基应用广泛。基于DMEM培养基中氨基酸、微生物含量丰富，并具有糖酵解中的丙酮酸和微量Fe离子，因此在细胞培养、疫苗生产等方面的应用为常见。
适用于细胞培养， 产品含4.5g/L 葡萄糖 ，含谷氨酰胺，不含丙酮酸钠。质量稳定可靠。
DMEM （Dulbecco's Modified Eagle Medium） （high Glucose）
with 4.5g/L Glucose，with L- glutamine，without sodium pyruvate
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细胞培养基 DMEM（H）（含双抗）