伯乐Immun-Star™ AP 化学发光试剂盒 | Bio-Rad Laboratories

Immun-Star™ AP 化学发光试剂盒 | Bio-Rad Laboratories

高度灵敏的 Immun-Star AP 试剂盒结合了 Bio-Rad 的印迹试剂和 CDP-Star 化学发光技术的专长。

描述

化学发光Western杂交检测,是同位素检测的一种高灵敏度的替代方法。酶标记抗体取代了放射性标记抗体,当它作用于底物时,可产生光信号。Luminol增强剂进一步加强信号输出,能检测到皮克级的靶蛋白,并把信号持续时间延长到 6–8 小时。发光信号可被胶片或者成像系统捕获,典型的曝光时间为30秒至5分钟。初始激发后光信号可持续24小时,因此用户可以进行多次曝光。Immun-Star™ 试剂盒是替代同位素检测的一种高灵敏度、快速的检测方法。

Immun-Star AP检测试剂盒结合了Bio-Rad 印迹试剂和 CDP-Star 化学发光技术的优点,其他特点包括:

  • 工作液在室温下保持 24 小时稳定性
  • 与 PVDF 和硝化纤维素膜兼容
  • 几个星期后,杂交印迹仍可通过加入新鲜化学发光底物而再活化
  • 能检测到10pg蛋白,灵敏度可与放射性方法媲美
  • 可以洗脱和重新杂交

提供两种基于羊抗小鼠或羊抗兔结合物的试剂盒。

 

Related Products

  • 硝酸纤维素膜,0.45 µm

  • HRP 偶联二抗

  • 硝酸纤维素膜,0.2 µm

技术指标

最低检测灵敏度
皮克

信号持续时间
24 小时

主要检测方法
压片

建议的抗体稀释比例
主要: 1/1,000–1/6,000
二抗: 1/3,000

推荐的膜
硝酸纤维素或 PVDF

保质期
4°C 下 12 个月

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Immun-Star™ AP 化学发光试剂盒 | Bio-Rad Laboratories

(停产) Immun-Star™ AP Substrate

1705018

125 ml, alkaline phosphatase substrate for 2,500 cm2 of membrane (~50 mini blots)

Immun-Star™ AP 化学发光试剂盒 | Bio-Rad Laboratories

(停产) Immun-Star™ Goat Anti-Mouse (GAM)-AP Detection Kit

1705010

Chemiluminescent western blot detection kit, includes substrate, enhancer, solutions, and AP secondary antibody conjugate; for 2,500 cm2 of membrane (~50 mini blots)

Immun-Star™ AP 化学发光试剂盒 | Bio-Rad Laboratories

(停产) Immun-Star™ Goat Anti-Rabbit (GAR)-AP Detection Kit

1705011

Chemiluminescent western blot detection kit, includes substrate, enhancer, solutions, and AP secondary antibody conjugate; for 2,500 cm2 of membrane (~50 mini blots)

Immun-Star™ AP 化学发光试剂盒 | Bio-Rad Laboratories

(停产) Immun-Star™ AP Substrate Pack

1705012

Pkg of 1, alkaline phosphatase substrates and enhancer for 2,500 cm2 of membrane (~50 mini blots)

Immun-Star™ AP 化学发光试剂盒 | Bio-Rad Laboratories

(停产) Goat Anti-Mouse (GAM)-AP Intro Kit

1705013

Chemiluminescent western blot detection kit, includes substrate, enhancer, solutions, and AP secondary antibody conjugate; for 400 cm2 of membrane (~8 mini blots)

Immun-Star™ AP 化学发光试剂盒 | Bio-Rad Laboratories

(停产) Goat Anti-Rabbit (GAR)-AP Intro Kit

1705014

Chemiluminescent western blot detection kit, includes substrate, enhancer, solutions, and AP secondary antibody conjugate, reagents; for 400 cm2 of membrane (~8 mini blots)

Immun-Star™ AP 化学发光试剂盒 | Bio-Rad Laboratories

(停产) Blotting Reagents Pack

1705015

Pkg of 1, TBS, Tween 20, nonfat dry milk for 2,500 cm2 of membrane (~50 mini blots)

相关资料

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Number 描述 Options

2032

Western Blotting Detection Reagents Brochure, Rev F

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4006074

Instruction Manual, Immun-Star Chemiluminescent Protein Detection System, Rev D

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