伯乐ProteoMiner 蛋白质浓缩试剂盒 | Bio-Rad Laboratories

ProteoMiner 蛋白质浓缩试剂盒 | Bio-Rad Laboratories

ProteoMiner 蛋白质浓缩技术可利用基于微珠的大型组合肽配体库,以便稀释高丰度蛋白和浓缩低丰度蛋白。

描述

ProteoMiner 蛋白质浓缩技术采用一个基于微球的高度多样化的大型组合肽配体库,可即时稀释高丰度蛋白和浓缩低丰度蛋白。 当对微球应用复合生物样本时,高丰度蛋白会浸透其高亲和性配体,并会有过量的蛋白质被冲洗掉。 相反,低丰度蛋白会在其特定的亲和配体上浓缩。

ProteoMiner 蛋白质浓缩试剂盒:

  • 减少高丰度蛋白的数量而不会导致免疫耗竭,从而防止损失与高丰度蛋白相结合的蛋白质。
  • 浓缩无法通过传统方法检测的中丰度和低丰度蛋白。
  • 不依赖预定义的抗体组,不同于免疫耗竭产品。
  • 与各种样本类型兼容。
  • 提供一种简便且易于使用的格式。
  • 可用于差异表达分析。

 

提供多种试剂盒
小容量试剂盒适用于处理 ≥10 mg 蛋白质,而大容量试剂盒适用于处理 ≥50 mg 蛋白质。 这些试剂盒已进行优化,适用于血清和血浆样品,但它们可用于推荐的总蛋白范围内的其他样品类型。

小容量和大容量试剂盒有两种形式:

  • ProteoMiner 蛋白质浓缩试剂盒 — 提供了分析低丰度蛋白的所有必需试剂,可在简单、易于执行的过程中使用单一洗脱剂。
  • ProteoMiner 连续洗脱试剂盒 — 利用多种洗脱试剂根据不同的性质对蛋白质进行连续洗脱。 这些试剂盒可分级分离蛋白质以改善检测和分辨率,并已针对 SELDI 试验样本(试剂不适合直接用于 2-D 凝胶电泳)进行了优化。

 

Related Technologies

  • Horizontal Streaking

Related Products

  • ReadyPrep™ 蛋白萃取试剂盒(细胞质/原子核)

  • ReadyPrep™ 萃取和增溶试剂

  • ProteoMiner 蛋白质浓缩试剂盒

  • ReadyPrep™ 蛋白萃取试剂盒(膜 I)

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ProteoMiner 蛋白质浓缩试剂盒 | Bio-Rad Laboratories

(停产) ProteoMiner™ Sequential Elution Reagents

1633003

10 preps, protein preparation reagents for eluting proteins into multiple fractions, columns not included, for use with #1633006 and #1633007

ProteoMiner 蛋白质浓缩试剂盒 | Bio-Rad Laboratories

(停产) ProteoMiner™ Protein Enrichment Small-Capacity Kit

1633006

10 preps, low-abundance protein enrichment kit for 10 mg of total protein, includes 10 spin columns, wash buffer, elution reagents, collection tubes

ProteoMiner 蛋白质浓缩试剂盒 | Bio-Rad Laboratories

(停产) ProteoMiner™ Protein Enrichment Large-Capacity Kit

1633007

10 preps, low-abundance protein enrichment kit for 50 mg of total protein, includes 10 spin columns, wash buffer, elution reagents, collection tubes

ProteoMiner 蛋白质浓缩试剂盒 | Bio-Rad Laboratories

(停产) ProteoMiner™ Protein Enrichment Introductory Small-Capacity Kit

1633008

2 preps, low-abundance protein enrichment kit for 10 mg of total protein, includes 2 spin columns, wash buffer, elution reagents, collection tubes

ProteoMiner 蛋白质浓缩试剂盒 | Bio-Rad Laboratories

(停产) ProteoMiner™ Protein Enrichment Introductory Large-Capacity Kit

1633009

2 preps, low-abundance protein enrichment kit for 50 mg of total protein, includes 2 spin columns, wash buffer, elution reagents, collection tubes

ProteoMiner 蛋白质浓缩试剂盒 | Bio-Rad Laboratories

(停产) ProteoMiner™ Sequential Elution Small-Capacity Kit

1633010

10 preps, protein preparation kit for sequential elution into 4 fractions, for 10 mg of total protein, includes 10 spin columns, buffers, collection tubes

ProteoMiner 蛋白质浓缩试剂盒 | Bio-Rad Laboratories

(停产) ProteoMiner™ Sequential Elution Large-Capacity Kit

1633011

10 preps, protein preparation kit for sequential elution into 4 fractions, for 50 mg of total protein, includes 10 spin columns, buffers, collection tubes

配套设备

ProteoMiner 蛋白质浓缩试剂盒 | Bio-Rad Laboratories

(停产) Mini Bio-Spin® Chromatography Columns

7326207

Pkg of 100, empty polypropylene spin columns, 1.2 ml bed volume, disposable, autoclavable

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Comparative Proteomics of Umbilical Cord and Maternal Plasma, Rev A

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5632

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Sample Preparation: Tools for Protein Sample Extraction, Cleanup, Fractionation, and Depletion Brochure, Rev B

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ProteoMiner Protein Enrichment Technology Bulletin, Rev B

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Instruction Manual, ProteoMiner Protein Enrichment Kits, Rev D

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ProteoMiner Protein Enrichment and Sequential Kit Protocols, Rev A

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2-D Analysis of Leaf Protein Samples Treated with ProteoMiner Beads Under Denaturing and Nondenaturing Conditions Poster, Rev A

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