伯乐CFX Connect™ 荧光定量 PCR 检测系统 | Bio-Rad Laboratories

CFX Connect 荧光定量 PCR 检测系统为双目标多重 PCR 和单荧光团试验的常规检测提供极佳的热性能。

描述

CFX Connect 荧光定量 PCR 检测系统可进行 2 个目标基因的分析、卓越的 PCR 仪技术以及与 CFX96 Touch™ 荧光定量 PCR 检测系统相同的可靠性能。系统将创新的光学技术与强大的软件相结合，因而能满足所有荧光定量 PCR 需求。

主要功能和优点

CFX Connect 荧光定量 PCR 检测系统可提供：

易于启动 — 使用经工厂校准的染料、快速设置和直观软件立即获得令人满意的结果
轻松优化 — 通过使用温度梯度在单次运行中优化检测，从而节省时间并降低成本。
强大的数据分析工具 — 使用 CFX Manager™ 软件的高级分析模块可快速并准确地验证和分析数据
加速出版物提交过程 — 包括 MIQE 注释，并使用 Biogazelle 的 qbase^PLUS 软件生成 RDML 文件

Related Technologies

What is High Resolution Melting (HRM)?
What is Real-Time PCR (qPCR)?
qPCR Assay Design and Optimization
MIQE and RDML Guidelines
Real-Time PCR Doctor | Gene Expression/Quantification Experiments
Introduction to PCR Primer & Probe Chemistries
Real-Time PCR Experimental Design

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技术指标

Chassis

CFX Connect

Maximum ramp rate, °C/sec

Average ramp rate, °C/sec

3.3

加热和冷却方法

Peltier

Lid, °C

Heats up to 105

温度

Range, °C

0–100

Accuracy, °C

±0.2 of programmed target at 90°C

Uniformity, °C

±0.4 well-to-well within 10 sec of arrival at 90°C

梯度

Operational range, °C

30–100

Programmable span, °C

1–24

光学检测

激励

3 个带滤光片的 LED

检测

3 个带滤光片的光敏二极管

激发/发射波长的范围，nm

450–580

Sensitivity

Detects 1 copy of target sequence in human genomic DNA

动态范围

10 数量级

扫描时间

所有通道，秒

FAM/SYBR^® Green only, sec

软件

操作系统

Windows XP，Windows Vista，Windows 7

多重分析

每孔最多 2 个目标基因

系统

已针对荧光定量 PCR 进行许可

是

样本载量，孔

Sample size, µl

1–50 (10–25 recommended)

通信接口

USB 2.0

电气认证

IEC，CE

尺寸（宽 x 长 x 高），cm/in

33 x 46 x 36/13 x 18 x 14

Weight, kg/lb

21/47

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items

Use the filters below to refine results!

(停产) CFX Connect Real-Time PCR Detection System

1855201

Two-color real-time PCR detection system, includes CFX Connect Thermal Cycler Chassis, CFX Connect Optical Reaction Module, cables

配套设备

(停产) CFX Qualification Plate

1845098

Predispensed 96-well plate for performance validation of CFX96™, CFX96 Touch™, CFX96 Touch™ Deep Well, or CFX Connect™ Real-Time PCR Detection System, includes template DNA, primers, supermix, water

(停产) PX1 PCR Plate Sealer

1814000

PCR plate sealer, includes heat sealing instrument, plate support block that holds 96-well and 384-well plates, sealing frame, power cord

试剂&试剂盒

(停产) SsoAdvanced™ Universal SYBR^® Green Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml)

1725271

5 ml (5 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl₂, SYBR^® Green I, ROX Normalization Dyes, for 500 x 20 µl reactions

(停产) SsoAdvanced Universal Probes Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml)

1725281

5 ml (5 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl₂, stabilizers, ROX Normalization Dyes, for 500 x 20 µl reactions

(停产) iTaq™ Universal SYBR^® Green Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml)

1725121

500 x 20 µl reactions, 5 ml (5 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl₂, SYBR^® Green I, enhancers, stabilizers, and a blend of passive reference dyes

(停产) iTaq™ Universal Probes Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml)

1725131

500 x 20 µl reactions, 5 ml (5 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl₂, enhancers, stabilizers, and ROX Normalization Dyes

(停产) SsoAdvanced™ PreAmp Supermix, 50 x 50 µl rxns, 1.25 ml

1725160

1.25 ml (1 x 1.25 ml), 2x PreAmp Mix contains dNTPs, Sso7d fusion polymerase, salts, enhancers, stabilizers, and other proprietary components, for 50 x 50 µl reactions

(停产) SingleShot™ SYBR^® Green Kit for Cell Lysis and RT-qPCR, 100 x 50 µl reactions

1725085

Pkg of 1, kit includes SingleShot Cell Lysis Reagents, iScript™ Advanced cDNA Synthesis Kit for RT-qPCR, SsoAdvanced™ Universal SYBR^® Green Supermix, and the SingleShot RNA Control, for 100 x 50 µl reactions

(停产) iScript™ Advanced cDNA Synthesis Kit, 100 x 20 µl rxns

1725038

100 x 20 µl reactions, includes 400 µl 5x iScript Advanced Reaction Mix, 100 µl iScript Advanced Reverse Transcriptase

(停产) iScript™ Reverse Transcription Supermix, 100 x 20 µl rxns, 400 µl

1708841

100 x 20 µl reactions, 4 x 100 µl 5x supermix, contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl₂, stabilizers; 50 reactions of no-RT control supermix

塑料耗材

(停产) Hard-Shell® 96-Well PCR Plates, low profile, thin wall, skirted, white/clear

HSP9601

Pkg of 50, white shell/clear well PCR plate, rigid 2-component design

(停产) Hard-Shell® 96-Well PCR Plates, low profile, thin wall, skirted, white/clear, barcoded

HSP9901

Pkg of 50, white shell/clear well PCR plate, barcoded, 7-digit sequential, Code 128, rigid 2-component design

The following replacement is recommended:

Hard-Shell® 96-Well PCR Plates, low profile, thin wall, skirted, white/white ( HSP9655 )

(停产) Hard-Shell® 96-Well PCR Plates, low profile, thin wall, skirted, white/white

HSP9655

Pkg of 50, white shell/white well PCR plate for higher fluorescent signals, rigid 2-component design

(停产) Multiplate™ 96-Well PCR Plates, low profile, unskirted, clear

MLL9601

Pkg of 25, clear PCR plate, one-component polypropylene, can be cut for fewer samples

(停产) Multiplate™ 96-Well PCR Plates, low profile, unskirted, white

MLL9651

Pkg of 25, white PCR plate, one-component polypropylene, can be cut for fewer samples

(停产) Microseal 'B' PCR Plate Sealing Film, adhesive, optical

MSB1001

Pkg of 100, optically clear seal for PCR plates

(停产) 0.2 ml 8-Tube PCR Strips without Caps, low profile, clear

TLS0801

Pkg of 120, thin-wall polypropylene 8-tube strips for PCR and low-volume reactions, clear (960 tubes)

(停产) 0.2 ml 8-Tube PCR Strips without Caps, low profile, white

TLS0851

Pkg of 120, thin-wall polypropylene 8-tube strips for PCR and low-volume reactions, white (960 tubes)

(停产) 0.2 ml Flat PCR Tube 8-Cap Strips, optical, ultraclear

TCS0803

Pkg of 120, 8-cap strips for 0.2 ml tubes and plates for PCR and optical reactions, ultraclear

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Precision Melt Analysis™ v1.2 Software Upgrade

SOFT-PM-12-PATCH

Upgrade Precision Melt Analysis Software from version 1.0 or 1.1 to version 1.2 for use with CFX Manager Software version 2.0 and higher

Download Software

Software Download

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