伯乐Profinia 蛋白质纯化系统试剂盒 | Bio-Rad Laboratories

Profinia 蛋白质纯化系统试剂盒 | Bio-Rad Laboratories

Profinia 试剂盒可用于多种蛋白质纯化方法,例如在 Profinia 蛋白质纯化系统中进行 IMAC、GST 和脱盐。

描述

Profinia 试剂盒可用于多种蛋白质纯化方法,例如运用 Profinia 系统进行 IMAC 纯化、GST 纯化以及蛋白脱盐等工作。Bio-Rad 提供了三类常用的试剂盒:

  • Profinia 纯化试剂盒
  • Profinia 启动试剂盒
  • Profinia 缓冲液试剂盒

Profinia 纯化试剂盒随附一组 Bio-Scale Mini 预装柱(用于 IMAC、GST 或脱盐)、特定应用的纯化缓冲液以及清洁和保存试剂。Profinia 启动试剂盒包含一组纯化预装柱以及特定应用的缓冲液和试剂,而且还附带对照裂解物以便于系统建立和最初的纯化。 如果您只需要补充缓冲液以及清洁和保存试剂,也可选择 Profinia 缓冲液试剂盒。

Profinia 耗材经过优化收集,易于使用而且最大限度地减少了纯化样品所需的时间。在 1ml 预装柱上单次纯化的典型产量和回收率如下:

  • 采用 Native IMAC,获得 ≥10 mg 蛋白质,用时约 35 分钟
  • 采用变性 IMAC,获得 ≥10 mg 蛋白质,用时约 20 分钟
  • 采用GST,获得 ≥10 mg 蛋白质,用时约 50 分钟

这些试剂盒中的所含的试剂均使用色谱级溶液配制,且预先用 0.2 µm 滤膜过滤。这些专门为Profinia系统配置的试剂可以使您自动运行平衡、结合、清洗和洗脱各步骤以及必要的预装柱维护程序(如清洁和保存)。

Related Products

  • Profinia 蛋白质纯化系统软件

  • Profinia 蛋白质纯化仪器

  • Profinia 蛋白质纯化系统附件

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Profinia 蛋白质纯化系统试剂盒 | Bio-Rad Laboratories

(停产) Profinia IMAC Purification Kit

6200225

Kit for use with the Profinia protein purification system, includes Profinia IMAC buffer kit (#620-0221), 2 each of 1 ml IMAC and 10 ml desalting cartridges

本产品不提供在线销售。欲了解更多信息,请联系客户服务。

Profinia 蛋白质纯化系统试剂盒 | Bio-Rad Laboratories

(停产) Profinia IMAC Purification Kit

6200235

Kit for use with the Profinia protein purification system, includes 1 each of 5 ml IMAC and 50 ml desalting cartridges, 2 Profinia IMAC buffer kits (#620-0221)

本产品不提供在线销售。欲了解更多信息,请联系客户服务。

Profinia 蛋白质纯化系统试剂盒 | Bio-Rad Laboratories

(停产) Profinia GST Purification Kit

6200226

Kit for use with the Profinia protein purification system, includes Profinia GST buffer kit (#620-0223), 2 each of 1 ml GST and 10 ml desalting cartridges

本产品不提供在线销售。欲了解更多信息,请联系客户服务。

Profinia 蛋白质纯化系统试剂盒 | Bio-Rad Laboratories

(停产) Profinia GST Purification Kit

6200236

Kit for use with the Profinia protein purification system, includes 1 each of 5 ml GST and 50 ml desalting cartridges, 2 Profinia GST buffer kits (#620-0223)

本产品不提供在线销售。欲了解更多信息,请联系客户服务。

Profinia 蛋白质纯化系统试剂盒 | Bio-Rad Laboratories

(停产) Profinia Desalting Purification Kit

6200228

Kit for use with the Profinia protein purification system, includes Profinia desalting buffer kit (#620-0224), 2 x 10 ml desalting cartridges

本产品不提供在线销售。欲了解更多信息,请联系客户服务。

Profinia 蛋白质纯化系统试剂盒 | Bio-Rad Laboratories

(停产) Profinia Desalting Purification Kit

6200238

Kit for use with the Profinia protein purification system, includes 50 ml desalting cartridge, 2 Profinia desalting buffer kits (#620-0224)

Profinia 蛋白质纯化系统试剂盒 | Bio-Rad Laboratories

(停产) Profinia IMAC Buffer Kit

6200221

Buffers and solutions sufficient for 10 applications, for use with the Profinia protein purification system

Profinia 蛋白质纯化系统试剂盒 | Bio-Rad Laboratories

(停产) Profinia GST Buffer Kit

6200223

Buffers, solutions, and glutathione sufficient for 10 applications, for use with the Profinia protein purification system

Profinia 蛋白质纯化系统试剂盒 | Bio-Rad Laboratories

(停产) Desalting and Cartridge Cleaning Buffer Kit

6200224

Buffers and solutions sufficient for 10 applications, for use with the Profinia protein purification system

Profinia 蛋白质纯化系统试剂盒 | Bio-Rad Laboratories

(停产) Profinia IMAC Starter Kit

6200229

Kit for use with the Profinia protein purification system, includes Profinia IMAC buffer kit (#620-0221), 1 each of 1 ml IMAC and 10 ml desalting cartridges, E. coli lysate

本产品不提供在线销售。欲了解更多信息,请联系客户服务。

Profinia 蛋白质纯化系统试剂盒 | Bio-Rad Laboratories

(停产) Profinia GST Starter Kit

6200230

Kit for use with the Profinia protein purification system, includes Profinia GST buffer kit (#620-0223), 1 each of 1 ml GST and 10 ml desalting cartridges, E. coli lysate, glutathione

本产品不提供在线销售。欲了解更多信息,请联系客户服务。

预装柱

Profinia 蛋白质纯化系统试剂盒 | Bio-Rad Laboratories

(停产) Bio-Scale Mini Profinity eXact Cartridge

7324648

Pkg of 1, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems

Profinia 蛋白质纯化系统试剂盒 | Bio-Rad Laboratories

(停产) Bio-Scale Mini Profinity eXact Cartridges

7324647

Pkg of 4, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems

Profinia 蛋白质纯化系统试剂盒 | Bio-Rad Laboratories

(停产) Bio-Scale Mini Profinity eXact Cartridges

7324646

Pkg of 2, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems

Profinia 蛋白质纯化系统试剂盒 | Bio-Rad Laboratories

(停产) Bio-Scale™ Mini Bio-Gel® P-6 Desalting Cartridges, 50 ml

7325314

Pkg of 5, 50 ml, prepacked gel filtration column, 80 mm x 28.2 mm, max. pressure 40 psi, for use with BioLogic™, Profinia™ and other chromatography systems

Profinia 蛋白质纯化系统试剂盒 | Bio-Rad Laboratories

(停产) Bio-Scale™ Mini Bio-Gel® P-6 Desalting Cartridge, 50 ml

7325312

Pkg of 1, 50 ml, prepacked gel filtration column, 80 mm x 28.2 mm, max. pressure 40 psi, for use with BioLogic™, Profinia™ and other chromatography systems

Profinia 蛋白质纯化系统试剂盒 | Bio-Rad Laboratories

(停产) Bio-Scale™ Mini Bio-Gel® P-6 Desalting Cartridges, 10 ml

7325304

Pkg of 5, 10 ml, prepacked gel filtration column, 80 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic™, Profinia™ and other chromatography systems

Profinia 蛋白质纯化系统试剂盒 | Bio-Rad Laboratories

(停产) Bio-Scale Mini Affi-Prep Protein A Cartridge

7324602

Pkg of 1, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

The following replacement is recommended:

  • EconoFit Affi-Prep Protein A Column, 1 x 5 ml ( 12009237 )
本产品不提供在线销售。欲了解更多信息,请联系客户服务。

Profinia 蛋白质纯化系统试剂盒 | Bio-Rad Laboratories

(停产) Bio-Scale Mini Affi-Prep Protein A Cartridges

7324600

Pkg of 5, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

The following replacement is recommended:

  • EconoFit Affi-Prep Protein A Columns, 5 x 1 ml ( 12009236 )
本产品不提供在线销售。欲了解更多信息,请联系客户服务。

Profinia 蛋白质纯化系统试剂盒 | Bio-Rad Laboratories

(停产) Bio-Scale Mini Profinity GST Cartridges

7324624

Pkg of 5, 5 ml, prepacked affinity columns, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

The following replacement is recommended:

  • EconoFit Profinity GST Columns, 5 x 5 ml ( 12009297 )
本产品不提供在线销售。欲了解更多信息,请联系客户服务。

Profinia 蛋白质纯化系统试剂盒 | Bio-Rad Laboratories

(停产) Bio-Scale Mini Profinity GST Cartridge

7324622

Pkg of 1, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

The following replacement is recommended:

  • EconoFit Profinity GST Column, 1 x 5 ml ( 12009296 )
本产品不提供在线销售。欲了解更多信息,请联系客户服务。

Profinia 蛋白质纯化系统试剂盒 | Bio-Rad Laboratories

(停产) Bio-Scale Mini Profinity GST Cartridges

7324620

Pkg of 5, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

The following replacement is recommended:

  • EconoFit Profinity GST Columns, 5 x 1 ml ( 12009295 )
本产品不提供在线销售。欲了解更多信息,请联系客户服务。

Profinia 蛋白质纯化系统试剂盒 | Bio-Rad Laboratories

(停产) Bio-Scale Mini Profinity IMAC Cartridges

7324614

Pkg of 5, 5 ml, prepacked Ni-charged column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

The following replacement is recommended:

  • EconoFit Profinity IMAC Columns, Ni-charged, 5 x 5 ml ( 12009300 )
本产品不提供在线销售。欲了解更多信息,请联系客户服务。

Profinia 蛋白质纯化系统试剂盒 | Bio-Rad Laboratories

(停产) Bio-Scale Mini Profinity IMAC Cartridge

7324612

Pkg of 1, 5 ml, prepacked Ni-charged column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

The following replacement is recommended:

  • EconoFit Profinity IMAC Column, Ni-charged, 1 x 5 ml ( 12009299 )
本产品不提供在线销售。欲了解更多信息,请联系客户服务。

Profinia 蛋白质纯化系统试剂盒 | Bio-Rad Laboratories

(停产) Bio-Scale Mini Profinity IMAC Cartridges

7324610

Pkg of 5, 1 ml, prepacked Ni-charged column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

The following replacement is recommended:

  • EconoFit Profinity IMAC Columns, Ni-charged, 5 x 1 ml ( 12009298 )
本产品不提供在线销售。欲了解更多信息,请联系客户服务。

相关资料

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Number 描述 Options

M10006044

Instruction Manual, Profinia Purification and Buffer Kits, Rev D

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M10006057

Instruction Manual, Profinia IMAC and GST Starter Kits, Rev E

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10015932

Profinia™ Protein Purification System FAQs, Rev B

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