伯乐Criterion XT Bis-Tris 预制胶 | Bio-Rad Laboratories

Criterion XT Bis-Tris 预制胶 | Bio-Rad Laboratories

Criterion XT 预制胶在近中性 PH 值条件下合成,与传统的 Laemmli 系统相比,具有更长的保质期,并提高了蛋白质的稳定性。

描述

Criterion™ XT 凝胶采用了优化的样品缓冲液和电泳缓冲液,无需添加抗氧化剂,是进行中型垂直电泳的理想选择, 和传统的 Laemmli 系统一样,Criterion XT 凝胶使用不连续缓冲体系先浓缩,后分离蛋白。

功能和优点

  • 保质期 12 个月
  • 使用 Bis-Tris-HCl 缓冲液系统 (pH 6.4) 配制,以按照分子量分离蛋白质
  • 从两种电泳缓冲液中进行选择,以扩展单个 Bis-Tris 凝胶类型的分离能力
  • 与传统的 Laemmli 系统相比,近中性 PH 值会大大延长丙烯酰胺水解的时间
  • 运行 2-D 凝胶只需不到一天的时间

应用和用途

  • 聚丙烯酰胺凝胶电泳(PAGE 或 SDS-PAGE)
  • 2-D 凝胶电泳分离
  • 样本筛选
  • 评估样本制备条件

如需进一步优化分辨率,请参阅蛋白质迁移图,以查看蛋白质在不同浓度凝胶中的迁移模式。

Related Products

  • 电泳缓冲液和试剂

  • PowerPac™ 通用电泳仪电源

  • Precision Plus Protein™ 预染标准品

  • Criterion™ 电泳槽

  • Criterion™ 转印设备

  • 上样缓冲液和试剂

技术指标

凝胶尺寸(宽 x 长 x 厚度)
13.3 x 8.7 x 0.1 厘米

凝胶夹尺寸(宽 x 长 x 厚度)
15.0 x 10.6 x 5.3 厘米

凝胶夹材料
苯乙烯共聚物

梳材料
聚碳酸酯

存储托盘材料
PET

凝胶贮存条件
存储温度保持在 2–8°C;不要冷冻

  在推荐温度下的凝胶保质期*
12 个月

缓冲液体积
上部,60 ml;下部,400 ml

Recommended sample buffer
XT sample buffer + XT reducing agent

推荐的电泳缓冲液
XT MES 或 XT MOPS

  凝胶化学
Bis-Tris

* 自生产日期起。
 

订购

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Criterion XT Bis-Tris 预制胶 | Bio-Rad Laboratories

(停产) 4–12% Criterion™ XT Bis-Tris Protein Gel, 12+2 well, 45 µl

3450123

Pkg of 1, 4–12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

Criterion XT Bis-Tris 预制胶 | Bio-Rad Laboratories

(停产) 4–12% Criterion™ XT Bis-Tris Protein Gel, 18 well, 30 µl

3450124

Pkg of 1, 4–12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

Criterion XT Bis-Tris 预制胶 | Bio-Rad Laboratories

(停产) 4–12% Criterion™ XT Bis-Tris Protein Gel, 26 well, 15 µl

3450125

Pkg of 1, 4–12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

Criterion XT Bis-Tris 预制胶 | Bio-Rad Laboratories

(停产) 4–12% Criterion™ XT Bis-Tris Protein Gel, Prep+2 well, 700 µl

3450126

Pkg of 1, 4–12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

Criterion XT Bis-Tris 预制胶 | Bio-Rad Laboratories

(停产) 10% Criterion™ XT Bis-Tris Protein Gel, 12+2 well, 45 µl

3450111

Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

Criterion XT Bis-Tris 预制胶 | Bio-Rad Laboratories

(停产) 10% Criterion™ XT Bis-Tris Protein Gel, 18 well, 30 µl

3450112

Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

Criterion XT Bis-Tris 预制胶 | Bio-Rad Laboratories

(停产) 10% Criterion™ XT Bis-Tris Protein Gel, 26 well, 15 µl

3450113

Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

Criterion XT Bis-Tris 预制胶 | Bio-Rad Laboratories

(停产) 12% Criterion™ XT Bis-Tris Protein Gel, 12+2 well, 45 µl

3450117

Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

Criterion XT Bis-Tris 预制胶 | Bio-Rad Laboratories

(停产) 12% Criterion™ XT Bis-Tris Protein Gel, 18 well, 30 µl

3450118

Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

Criterion XT Bis-Tris 预制胶 | Bio-Rad Laboratories

(停产) 12% Criterion™ XT Bis-Tris Protein Gel, 26 well, 15 µl

3450119

Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

Criterion XT Bis-Tris 预制胶 | Bio-Rad Laboratories

(停产) 12% Criterion™ XT Bis-Tris Protein Gel, Prep+2 well, 700 µl

3450120

Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

Criterion XT Bis-Tris 预制胶 | Bio-Rad Laboratories

(停产) 4–12% Criterion™ XT Bis-Tris Protein Gel, 11 cm IPG/prep+1 well, 550 µl

3450127

Pkg of 1, 4–12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

Criterion XT Bis-Tris 预制胶 | Bio-Rad Laboratories

(停产) 10% Criterion™ XT Bis-Tris Protein Gel, 11 cm IPG/prep+1 well, 550 µl

3450115

Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

Criterion XT Bis-Tris 预制胶 | Bio-Rad Laboratories

(停产) 12% Criterion™ XT Bis-Tris Protein Gel, 11 cm IPG/prep+1 well, 550 µl

3450121

Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

配套设备

Criterion XT Bis-Tris 预制胶 | Bio-Rad Laboratories

(停产) Mini-PROTEAN® Cassette Opening Lever

4560000

Pkg of 1, 100% aluminum and recyclable opening lever for Mini-PROTEAN precast gel cassettes

试剂&试剂盒

Criterion XT Bis-Tris 预制胶 | Bio-Rad Laboratories

(停产) XT MOPS Running Buffer

1610788

Pkg of 1, 500 ml, electrophoresis buffer for use with Criterion™ XT Bis-Tris Gels

Criterion XT Bis-Tris 预制胶 | Bio-Rad Laboratories

(停产) XT MES Running Buffer

1610789

Pkg of 1, 500 ml, 20x electrophoresis buffer for use with Criterion™ XT Bis-Tris Gels

Criterion XT Bis-Tris 预制胶 | Bio-Rad Laboratories

(停产) 20x XT MOPS Running Buffer Kit

1610793

Pkg of 1, electrophoresis buffer kit, includes 500 ml XT MOPS Running Buffer, 10 ml 4x XT Sample Buffer, 1 ml XT Reducing Agent

Criterion XT Bis-Tris 预制胶 | Bio-Rad Laboratories

(停产) XT MES Buffer Kit

1610796

Pkg of 1, electrophoresis buffer kit, includes 500 ml 20x XT MES Running Buffer, 10 ml 4x XT Sample Buffer, 1 ml 20x XT Reducing Agent

Criterion XT Bis-Tris 预制胶 | Bio-Rad Laboratories

(停产) XT Sample Buffer

1610791

10 ml, 4x premixed protein sample buffer for use with all Criterion XT gels

Criterion XT Bis-Tris 预制胶 | Bio-Rad Laboratories

(停产) XT Reducing Agent

1610792

1 ml, 20x reducing agent for use with all Criterion XT gels

相关资料

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Number 描述 Options

6040

Electrophoresis Guide, Interactive PDF, Rev C

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2788

Criterion Blotting Tray Insert, Rev A

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2912

Criterion XT Precast Gel Flier, Rev A

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2710

Criterion™ Precast Gel System Brochure, Rev C

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2911

Criterion XT Precast Gels Product Information Sheet, Rev B

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4110001

Instruction Manual, Criterion™ Gel Application Guide, Ver H

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4110130

Instruction Manual, Criterion XT Precast Gel Instruction Guide, Rev B

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