Criterion XT 预制胶在近中性 PH 值条件下合成,与传统的 Laemmli 系统相比,具有更长的保质期,并提高了蛋白质的稳定性。
描述
Criterion™ XT 凝胶采用了优化的样品缓冲液和电泳缓冲液,无需添加抗氧化剂,是进行中型垂直电泳的理想选择, 和传统的 Laemmli 系统一样,Criterion XT 凝胶使用不连续缓冲体系先浓缩,后分离蛋白。
功能和优点
- 保质期 12 个月
- 使用 Bis-Tris-HCl 缓冲液系统 (pH 6.4) 配制,以按照分子量分离蛋白质
- 从两种电泳缓冲液中进行选择,以扩展单个 Bis-Tris 凝胶类型的分离能力
- 与传统的 Laemmli 系统相比,近中性 PH 值会大大延长丙烯酰胺水解的时间
- 运行 2-D 凝胶只需不到一天的时间
应用和用途
- 聚丙烯酰胺凝胶电泳(PAGE 或 SDS-PAGE)
- 2-D 凝胶电泳分离
- 样本筛选
- 评估样本制备条件
如需进一步优化分辨率,请参阅蛋白质迁移图,以查看蛋白质在不同浓度凝胶中的迁移模式。
Related Products
-
-
-
Precision Plus Protein™ 预染标准品
-
-
-
技术指标
凝胶尺寸(宽 x 长 x 厚度)
13.3 x 8.7 x 0.1 厘米
凝胶夹尺寸(宽 x 长 x 厚度)
15.0 x 10.6 x 5.3 厘米
凝胶贮存条件
存储温度保持在 2–8°C;不要冷冻
Recommended sample buffer
XT sample buffer + XT reducing agent
推荐的电泳缓冲液
XT MES 或 XT MOPS
订购
items
Use the filters below to refine results!
(停产) 4–12% Criterion™ XT Bis-Tris Protein Gel, 12+2 well, 45 µl
3450123
Pkg of 1, 4–12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
(停产) 4–12% Criterion™ XT Bis-Tris Protein Gel, 18 well, 30 µl
3450124
Pkg of 1, 4–12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
(停产) 4–12% Criterion™ XT Bis-Tris Protein Gel, 26 well, 15 µl
3450125
Pkg of 1, 4–12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
(停产) 4–12% Criterion™ XT Bis-Tris Protein Gel, Prep+2 well, 700 µl
3450126
Pkg of 1, 4–12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
(停产) 10% Criterion™ XT Bis-Tris Protein Gel, 12+2 well, 45 µl
3450111
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
(停产) 10% Criterion™ XT Bis-Tris Protein Gel, 18 well, 30 µl
3450112
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
(停产) 10% Criterion™ XT Bis-Tris Protein Gel, 26 well, 15 µl
3450113
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
(停产) 12% Criterion™ XT Bis-Tris Protein Gel, 12+2 well, 45 µl
3450117
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
(停产) 12% Criterion™ XT Bis-Tris Protein Gel, 18 well, 30 µl
3450118
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
(停产) 12% Criterion™ XT Bis-Tris Protein Gel, 26 well, 15 µl
3450119
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
(停产) 12% Criterion™ XT Bis-Tris Protein Gel, Prep+2 well, 700 µl
3450120
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
(停产) 4–12% Criterion™ XT Bis-Tris Protein Gel, 11 cm IPG/prep+1 well, 550 µl
3450127
Pkg of 1, 4–12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
(停产) 10% Criterion™ XT Bis-Tris Protein Gel, 11 cm IPG/prep+1 well, 550 µl
3450115
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
(停产) 12% Criterion™ XT Bis-Tris Protein Gel, 11 cm IPG/prep+1 well, 550 µl
3450121
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
配套设备
(停产) Mini-PROTEAN® Cassette Opening Lever
4560000
Pkg of 1, 100% aluminum and recyclable opening lever for Mini-PROTEAN precast gel cassettes
试剂&试剂盒
(停产) XT MOPS Running Buffer
1610788
Pkg of 1, 500 ml, electrophoresis buffer for use with Criterion™ XT Bis-Tris Gels
(停产) XT MES Running Buffer
1610789
Pkg of 1, 500 ml, 20x electrophoresis buffer for use with Criterion™ XT Bis-Tris Gels
(停产) 20x XT MOPS Running Buffer Kit
1610793
Pkg of 1, electrophoresis buffer kit, includes 500 ml XT MOPS Running Buffer, 10 ml 4x XT Sample Buffer, 1 ml XT Reducing Agent
(停产) XT MES Buffer Kit
1610796
Pkg of 1, electrophoresis buffer kit, includes 500 ml 20x XT MES Running Buffer, 10 ml 4x XT Sample Buffer, 1 ml 20x XT Reducing Agent
(停产) XT Sample Buffer
1610791
10 ml, 4x premixed protein sample buffer for use with all Criterion XT gels
(停产) XT Reducing Agent
1610792
1 ml, 20x reducing agent for use with all Criterion XT gels
相关资料
Login Required
Number 描述 Options
6040
Electrophoresis Guide, Interactive PDF, Rev C
Log In to download this document
2788
Criterion Blotting Tray Insert, Rev A
Log In to download this document
2912
Criterion XT Precast Gel Flier, Rev A
Log In to download this document
2710
Criterion™ Precast Gel System Brochure, Rev C
Log In to download this document
2911
Criterion XT Precast Gels Product Information Sheet, Rev B
Log In to download this document
4110001
Instruction Manual, Criterion™ Gel Application Guide, Ver H
Log In to download this document
4110130
Instruction Manual, Criterion XT Precast Gel Instruction Guide, Rev B
Log In to download this document