iQ™ SYBR® 超混合液用于通过方便的 SYBR® Green I 检测化学方法，获得各种 实时 PCR 应用的最佳结果。该预混双倍溶液含有最佳浓度的 SYBR® Green I 染料（用于灵敏精确检测）、热启动 iTaq™ DNA 聚合酶、专有缓冲液和稳定剂（已专门针对使用 SYBR® Green I 的 qPCR 进行优化）和适合进行 qPCR 的 dNTP。此试剂盒还包含荧光素，这种物质作为惰性参比染料用于在某些 实时 PCR 检测系统上进行孔因子收集。使用 iQ™ SYBR® Green 试剂盒可最大程度地缩短制备时间、产生可靠的 实时 PCR 检测结果，并在六个数量级内提供精确的线性检测。
主要功能和优点
提高通量 — 因为仅需要模板和引物，所以所需准备时间极少
高灵敏度和精度 — 抗体介导的热启动 iTaq DNA 聚合酶最大限度地减少了非特定 PCR 产物的扩增，并可以对少至 10 个模板副本进行灵敏准确的检测
(停产) iQ™ SYBR® Green Supermix, 100 x 50 µl rxns, 2.5 ml (2 x 1.25 ml)
1708880
2.5 ml (2 x 1.25 ml), 2x qPCR mix, contains dNTPs, iTaq™ DNA Polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, for 100 x 50 µl reactions
(停产) iQ™ SYBR® Green Supermix, 500 x 50 µl rxns, 12.5 ml (10 x 1.25 ml)
1708882
12.5 ml (10 x 1.25 ml), 2x qPCR mix, contains dNTPs, iTaq™ DNA Polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, for 500 x 50 µl reactions
(停产) iQ™ SYBR® Green Supermix, 1,000 x 50 µl rxns, 25 ml (20 x 1.25 ml)
1708884
25 ml (20 x 1.25 ml), 2x qPCR mix, contains dNTPs, iTaq™ DNA Polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, for 1,000 x 50 µl reactions
(停产) iQ™ SYBR® Green Supermix, 2,000 x 50 µl rxns, 50 ml (1 x 50 ml)
1708885
50 ml (1 x 50 ml), 2x qPCR mix, contains dNTPs, iTaq™ DNA Polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, for 2,000 x 50 µl reactions
(停产) iQ™ SYBR® Green Supermix, 1,000 x 50 µl rxns, 25 ml (5 x 5 ml)
1708886
25 ml (5 x 5 ml), 2x qPCR mix, contains dNTPs, iTaq™ DNA Polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, for 1,000 x 50 μl reactions
(停产) iQ™ SYBR® Green Supermix, 2,000 x 50 µl rxns, 50 ml (10 x 5 ml)
1708887
50 ml (10 x 5 ml), 2x qPCR mix, contains dNTPs, iTaq™ DNA Polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, for 2,000 x 50 µl reactions
相关资料
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Number描述Options
4106212
Instruction Manual, iQ SYBR Green Supermix, 100 x 50 µl, Rev D
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10016680
iQ™ SYBR® Green Supermix User Guide, Rev A
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2764
iQ Supermixes for qPCR Flier, Rev E
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6252
Reagent Comparison Guide for Real-Time PCR Brochure, Rev C
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7178
Amplification: Real-Time PCR Reagents and Kits Brochure
