伯乐CHT Ceramic Hydroxyapatite XT Media | Bio-Rad Laboratories

CHT XT Media is engineered with advanced technology, resulting in unique selectivity and robust, uniformly-sized beads with excellent pressure-flow properties. It is fine-tuned for the removal of aggregates and process impurities.

描述

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CHT Ceramic Hydroxyapatite XT Media (CHT XT) is a calcium affinity cation exchange chromatography media. It is engineered with advanced technology, resulting in unique selectivity and robust, uniformly-sized beads with excellent pressure-flow properties. The chemical composition of CHT XT is (Ca₁₀(PO₄)₆(OH)₂) and it is a mixed-mode media. Biomolecules can interact with CHT through calcium affinity interactions and/or cation exchange interactions. It provides unparalleled separation properties and resolution. It can be used in applications such as purification of enzymes, monoclonal and polyclonal antibodies of various classes, antibody fragments, and bispecific antibodies; efficient isolation and purification of viruses and virus-like particles (VLPs); and separation of supercoiled DNA from linear duplexes as well as single stranded from double-stranded DNA. It is ideal for large scale bioprocess applications.

CHT XT Media delivers

Unique selectivity
Efficient single-step clearance of aggregates and other impurities
High physical and chemical stability
Straightforward column packing

Learn Best Practices and Guidelines for Packing CHT Media

These guidelines for handling and packing are recommended for all CHT users to ensure reproducible and high-quality results. Please download the CHT Application Guide for specific details on recommended buffers and slurry calculations.

Watch Videos | Download Application Guide

Product Formats

Product	Catalog Number
CHT XT Media, 10 g	12002457
CHT XT Media, 100 g	12002454
CHT XT Media, 1 kg	12002456
CHT XT Media, 5 kg	12002455

Screening Tools

Foresight CHT XT Column, 1 ml

12003150
Foresight CHT XT Column, 5 ml

12003149
Foresight CHT XT, 200 μl Robocolumn

12003152
Foresight CHT XT, 600 μl Robocolumn

12003148
Foresight CHT XT, 20 μl Plates

12003151

技术指标

Property

Description

Function

Mixed-mode: cation exchange (phosphate) and affinity (calcium)

官能团

Ca²⁺, PO₄, OH

Median particle size

40 ± 4 µm

Dynamic binding capacity

17–25 mg Lysozyme/g*
>60 mg/ml mAb G** (pI 9.2)
* At 500 cm/hr with 10 mM sodium phosphate, pH 6.8.
** At 100 cm/hr with 5 mM sodium phosphate, 25 mM sodium chloride, pH 7 in a 0.5 x 9.5 cm column

Recommended linear flow rate

50–300 cm/hr
Tested on a 0.4 x 10 cm column packed at 150 cm/hr

最高操作压力

10 bar (150 psi)

Tap settled density

0.67 g/ml

Compression factor

Incompressible

pH stability

6.5–14

Shipping form

Dry powder

再生

0.4 to 0.5 M sodium phosphate, pH 7.0-7.5; 1 M trisodium phosphate, pH 11-12. If higher concentrations of sodium phosphate are needed, use 0.4-1.0 M potassium phosphate.

Sanitization

1-2 N sodium hydroxide

储存条件

0.1 N sodium hydroxide

高压灭菌

121°C, 20 min in phosphate buffered saline, pH 7

化学兼容性

1 N sodium hydroxide, 8 M Urea, 6 M guanidine hydrochloride, ethanol, methanol, 100% acetonitrile, 0.4–1.0 M phosphate buffer, 0.5 M sodium phosphate, pH 7, 1.0 M trisodium phosphate, pH 11–12

保质期

5 years

订购

items

Use the filters below to refine results!

(停产) EconoFit CHT XT, 40 μm Column, 1 x 1 ml

12009261

Pkg of 1, 1 ml, prepacked mixed mode column, 7 mm x 25 mm, max. pressure 72 psi, for use with NGC Systems or any chromatography system

(停产) CHT™ Ceramic Hydroxyapatite XT, 40 µm

12002457

Pkg of 1, 10 g CHT XT mixed-mode chromatography media, 40 µm particle size

(停产) CHT™ Ceramic Hydroxyapatite XT, 40 µm

12002454

Pkg of 1, 100 g CHT XT mixed-mode chromatography media, 40 µm particle size

(停产) CHT Ceramic Hydroxyapatite XT, 40 µm, 1 kg

12002456

Pkg of 1, 1 kg CHT XT mixed-mode chromatography media, 40 µm particle size

(停产) CHT™ Ceramic Hydroxyapatite XT, 40 µm

12002455

Pkg of 1, 5 kg CHT XT mixed-mode chromatography media, 40 µm particle size

(停产) Foresight™ CHT™ XT Column, 5 ml

12003149

Pkg of 1, 5 ml column prepacked with CHT Ceramic Hydroxyapatite XT Mixed-Mode Chromatography Media, 40 µm particle size

(停产) Foresight™ CHT™ XT Column

12003150

Pkg of 1, 1 ml column prepacked with CHT Ceramic Hydroxyapatite XT Mixed-Mode Chromatography Media, 40 µm particle size

(停产) Foresight™ CHT™ XT Plates, 20 µl

12003151

2 x 96-well plates, 20 µl, filter plate prepacked with CHT Ceramic Hydroxyapatite XT Mixed-Mode Chromatography Media, 40 µm particle size

(停产) Foresight™ CHT™ XT RoboColumn, 200 µl

12003152

Pkg of 1, row of 8 columns, 200 µl, RoboColumn unit prepacked with CHT Ceramic Hydroxyapatite XT Mixed-Mode Media, 40 µm particle size

(停产) Foresight™ CHT™ XT RoboColumn, 600 µl

12003148

Pkg of 1, row of 8 columns, 600 µl, RoboColumn unit prepacked with CHT Ceramic Hydroxyapatite XT Mixed-Mode Media, 40 µm particle size

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伯乐CHT Ceramic Hydroxyapatite XT Media | Bio-Rad Laboratories

描述

Free Ceramic Hydroxyapatite reference wall charts for process purification

CHT XT Media delivers

Learn Best Practices and Guidelines for Packing CHT Media

Product Formats

Screening Tools

Foresight CHT XT Column, 1 ml

Foresight CHT XT Column, 5 ml

Foresight CHT XT, 200 μl Robocolumn

Foresight CHT XT, 600 μl Robocolumn

Foresight CHT XT, 20 μl Plates

技术指标

订购

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