伯乐Bio-Plex Pro Human Th17 Cytokine Assays | Bio-Rad Laboratories

Magnetic bead–based assays for the detection and measurement of 16 human Th17 biomarkers. Available in a premixed, all-in-one 15-plex assay, singleplex sets, or as custom x-Plex and Express Assays.

描述

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The Bio-Plex Pro Human Th17 Cytokine Panel enables measurement of the following analytes in diverse matrices such as serum, plasma, and cell culture supernatants:

IL-1β
IL-4
lL-6
IL-10
IL-17A
IL-17F
IL-17A/F (singleplex only)
IL-21
IL-22
IL-23
IL-25
IL-31
IL-33
IFN-γ
sCD40L
TNF-α

Based on 6.5 µm magnetic beads, these assays are compatible with both vacuum and magnetic wash stations. Magnetic washing simplifies plate processing, provides increased throughput, and results in decreased variability and increased reproducibility.

The multiplex format enables robust and reproducible measurement of 15+1 soluble proteins involved in the T-helper cell type 17 immune response pathway, which is thought to play a key role in inflammation-associatied conditions such as antimicrobial immunity, cancer, transplant rejection, and autoimmune diseases including rheumatoid arthritis, multiple sclerosis, lupus and psoriasis.

Reagent kit ll with diluents optimized for serum and plasma samples
Assay quick guide to get you started right away
Requires as little as 12.5 µl of sample for both multiplex and singleplex assays
Includes premixed standards for generation of standard curves over a broad dynamic range for each target
2-level quality controls included with premixed kits

Flexible Ordering Options

Premixed panel — complete, ready-to-use 15-plex Bio-Plex Pro Human Th17 Cytokine Panel and 3-plex Bio-Plex Pro TGF-β Assay Kits
Human Th17 singleplex sets — each set includes a vial of coupled magnetic beads and a vial of detection antibodies; requires Reagent Kit III (171304090M) and a vial of standards (171DA0001)
Custom premixed assays (We Mix) — includes 2-level quality controls, available as Bio-Plex x-Plex Assays built using the Bio-Plex Assay Builder
Custom assays (You Mix) — available as Bio-Plex Express Assays built using the Bio-Plex Assay Builder

Additional Th17 Pathway Assays:

TGF-β 3-plex panel (171V4003M)
TGF-β singleplex sets — each set includes a vial of coupled magnetic beads and a vial of detection antibodies; requires a reagent kit (171304090M) for magnetic separation and a vial of standards (171D60001) for a complete assay

Instrument Compatibility

Compatible with these readers and wash stations:

Bio-Plex MAGPIX or Luminex MAGPIX System
Bio-Plex 200 or Luminex 200 System
Bio-Plex 3D or Luminex FLEXMAP 3D System
Bio-Plex Pro Wash Stations

More Information

See how Bio-Plex Instruments and Assays benefit researchers studying heart disease, leukemia, melanoma, and other cancers.

Related Products

Bio-Plex Pro Human Inflammation Assays
For the first time, the key biomarkers of inflammation from the TNF superfamily proteins, IFN family proteins, Treg cytokines, and MMPs can be measured using a single multiplex kit. Bio-Plex Pro Human Inflammation Assays are available in larger 24-plex and 37-plex screening panels and a pathway-focused Treg 12-plex panel, along with singleplex and custom-configured assays.

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(停产) Bio-Plex Pro™ Human Th17 Cytokine Panel 15-Plex

171AA001M

1 x 96-well all-in-one kit, includes coupled magnetic beads, detection antibodies, standards, controls, buffers, diluents, streptavidin-PE, 96-well flat bottom plate, and sealing tape, for the detection of 15 human Th17 cytokines

配套设备

(停产) Bio-Plex Pro™ Wash Station

30034376

Microplate wash station for magnetic bead–based assays, includes magnetic plate carrier, waste bottle, 2 buffer bottles

(停产) Bio-Plex® Handheld Magnetic Washer

171020100

Includes magnetic washer and adjustment hex tools for use in manual wash steps for all Bio-Plex Magnetic Assays

(停产) Filter Plate

171304502

Pkg of 1, 96-well plate, with clear plastic lid and tray, for Bio-Plex Assays using the vacuum wash method, sealing tape not included

(停产) Bio-Plex® Wash Buffer

171304500

1.5 L, wash buffer for use with Bio-Plex Pro wash stations; not for use with phosphoprotein assays

(停产) Bio-Plex Pro™ Flat Bottom Plates

171025001

Pkg of 40, 96-well plate, for use with Bio-Plex Pro wash stations when using magnetic bead–based assays

Rgnts & Stds

(停产) Bio-Plex Pro Reagent Kit III with Flat Bottom Plate, 1 x 96-well

171304090M

1 x 96-well, includes detection antibody diluent HB, standard diluent HB, sample diluent HB, assay buffer, wash buffer, streptavidin-PE, flat bottom plate, and sealing tape, for magnetic separation methods

(停产) Bio-Plex Pro Human Th17 Standards, 50 x 96 assays

171DA0501

Pkg of 50 lot-matched vials, lyophilized mixture of 16 standard antigens, enough for 50 x 96 assays; for use with Human Th17 Assays and Reagent Kit III (171304090M)

(停产) Bio-Plex Pro TGF-β Standards, 96 assays

171X40001

Pkg of 1 vial, lyophilized TGF-β standard for detecting TGF-β1, TGF-β2, and TGF-β3 analytes, enough for 96 assays; for use with TGF-β Assays and Reagent Kit III (171304090M)

(停产) Bio-Plex Pro TGF-β Standards, 50 x 96 assays

171X40501

Pkg of 50 lot-matched vials, lyophilized TGF-β standard for detecting TGF-β1, TGF-β2, and TGF-β3 analytes, enough for 50 x 96 assays; for use with TGF-β Assays and Reagent Kit III (171304090M)

Singleplexes

(停产) Bio-Plex Pro Human Th17 Cytokine IL-1β Set

171BA001M

1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-1β. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Th17 Standards (171DA0001), which are not included.

(停产) Bio-Plex Pro Human Th17 Cytokine lL-4 Set

171BA002M

1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-4. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Th17 Standards (171DA0001), which are not included.

(停产) Bio-Plex Pro Human Th17 Cytokine lL-6 Set

171BA003M

1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human lL-6. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Th17 Standards (171DA0001), which are not included.

(停产) Bio-Plex Pro Human Th17 Cytokine IL-10 Set

171BA004M

1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-10. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Th17 Standards (171DA0001), which are not included.

(停产) Bio-Plex Pro Human Th17 Cytokine IL-17A Set

171BA005M

1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-17A. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Th17 Standards (171DA0001), which are not included.

(停产) Bio-Plex Pro Human Th17 Cytokine IL-17F Set

171BA006M

1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-17F. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Th17 Standards (171DA0001), which are not included.

(停产) Bio-Plex Pro Human Th17 Cytokine IL-21 Set

171BA007M

1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-21. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Th17 Standards (171DA0001), which are not included.

(停产) Bio-Plex Pro Human Th17 Cytokine IL-22 Set

171BA008M

1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-22. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Th17 Standards (171DA0001), which are not included.

(停产) Bio-Plex Pro Human Th17 Cytokine IL-23 Set

171BA009M

1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-23. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Th17 Standards (171DA0001), which are not included.

(停产) Bio-Plex Pro Human Th17 Cytokine IL-25 Set

171BA010M

1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-25. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Th17 Standards (171DA0001), which are not included.

(停产) Bio-Plex Pro Human Th17 Cytokine IL-31 Set

171BA011M

1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-31. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Th17 Standards (171DA0001), which are not included.

(停产) Bio-Plex Pro Human Th17 Cytokine IFN-γ Set

171BA013M

1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IFN-γ. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Th17 Standards (171DA0001), which are not included.

(停产) Bio-Plex Pro Human Th17 Cytokine sCD40L Set

171BA014M

1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human sCD40L. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Th17 Standards (171DA0001), which are not included.

(停产) Bio-Plex Pro Human Th17 Cytokine TNF-α Set

171BA015M

1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human TNF-&alpha. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Th17 Standards (171DA0001), which are not included.

(停产) Bio-Plex Pro Human Th17 Cytokine IL-17A/F Set

171BA016M

1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-17A/F. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of Human Th17 Standards (171DA0001), which are not included.

(停产) Bio-Plex Pro TGF-β1 Set

171V4001M

1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting TGF-β1. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of TGF-β Standards (171X40001), which are not included.

(停产) Bio-Plex Pro TGF-β2 Set

171V4002M

1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting TGF-β2. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of TGF-β Standards (171X40001), which are not included.

(停产) Bio-Plex Pro TGF-β3 Set

171V4003M

1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting TGF-β3. Requires Reagent Kit III (171304090M) for magnetic separation, and 1 vial of TGF-β Standards (171X40001), which are not included.

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