伯乐S3e Cell Sorter | Bio-Rad Laboratories

The S3e Cell Sorter is enhanced with the AutoGimbal System, which enables hands-free motorized nozzle tip alignment. Real-time automation allows walk-away cell sorting.

描述

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The S3e Cell Sorter is the first truly walk-away automated cell sorter. Real-time monitoring and smart features make cell sorting easier and accessible to both novices and experts.

This compact system is equipped with either one to three lasers and up to four fluorescence detectors plus forward- and side-scatter detectors.

Features and Benefits of the S3e Cell Sorter

Hands-free nozzle tip alignment — features the fully automatic AutoGimbal System, driven completely by software
Simplified instrument setup — fully automated drop delay calculation, stream alignment, and droplet break-off monitoring enable precise 1–4 color sorting with minimal training
Compact design — dimensions of only 70 × 65 × 65 cm (2.3 × 2.1 × 2.1 ft) including onboard fluidics and temperature control system
2-way cell sorting — sort two different defined populations at the same time
High speed and high purity — sort cells fast while maintaining high sensitivity and purity
Intuitive interface — user-friendly ProSort™ Software enables effortless instrument control and sort logic definition

Among the many innovations of the S3e Cell Sorter, two key state-of-the-art advancements are ProDrop Technology and the AutoGimbal System:

ProDrop Technology produces extremely accurate and precise results by:

Automating drop delay calculation and droplet break-off monitoring
Allowing drop delay calculation through direct counting of bead events
Simplifying one of the most complex and error-prone aspects of cell sorting

The highly sophisticated AutoGimbal System automates alignment of the nozzle tip and stream to optics by:

Providing hands-free alignment at all points on the stream and consistency in stream position from day to day and user to user
Combining picomotor fine motion positioning, imaging, and response to software algorithms using camera images

The affordable S3e Cell Sorter provides exceptional sort purity, recovery, and yield without compromising performance and sensitivity.

The system is ideal for sorting cells expressing fluorescent proteins such as eGFP, DsRed, and mCherry, or for cells labeled with fluorescent markers such as antibodies labeled with PE, PE tandems, and other fluorophores excited by the 405, 488, 561, and 640 nm lasers.

Related Technologies

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Stem Cell Research

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技术指标

Droplet frequency

37–43 kHz

Sorting type

True jet-in-air for high-performance sorting

Sorting rate

No hardware limitations for sort rate, limited only by droplet frequency and application

Sorting purity

>99% pure

Nozzle size

100 µm

Sorting direction

2-way sorting

Sorting collection

Up to 5 x 5 ml sample tubes each direction
Up to 5 x 1.5 ml tubes each direction
Microscope slides
8-well strip each direction

Lasers

Single-laser: 488 nm 100 mW
Single-laser upgrade: 488 nm 100 mW plus 561 or 640 nm 100 mW
Dual-laser: 488 and 561 or 640 nm 100 mW
Tri-laser: 405, 488, and 561 or 405, 488, and 640 or 488, 561, and 640 mW 100 mW

检测

Forward scatter (FSC) with PMT
Side scatter (SSC) with PMT
Up to 4 fluorescence detectors with PMT
Minimum resolution: 0.5 µm

灵敏度

<125 MESF for FITC and PE

温度范围

Sample and collection temperature control system: 4–37°C
Peltier solid state system

Fluidics

Onboard fluidics and dilution of 8x sheath fluid with deionized water

Data format

FCS 3.1

尺寸

70 x 65 x 65 cm (2.3 x 2.1 x 2.1 ft)

订购

items

Use the filters below to refine results!

(停产) S3e™ Cell Sorter (488 nm)

1451005

S3e Cell Sorter, 488 nm 100 mW laser, includes 2 fluorescence detectors with filters, AutoGimbal™ System, ProSort™ Software

(停产) S3e™ Cell Sorter (488/561 nm)

1451006

S3e Cell Sorter, 488 and 561 nm 100 mW lasers, includes 4 fluorescence detectors with filters, AutoGimbal™ System, ProSort™ Software

(停产) S3e™ Biosafety System Class I

1451078

Biosafety System Class I for S3™ and S3e Cell Sorters, includes HEPA filter, fan blower, base, aluminum frame, 30 mil vinyl panels with magnetic attachment

(停产) S3e™ Cell Sorter (488 nm) with S3e Biosafety System Class I

1451029

S3e Cell Sorter, 488 nm 100 mW laser, 2 fluorescence detectors, with S3e Biosafety System Class I

(停产) S3e™ Cell Sorter (488/561 nm) with S3e Biosafety System Class I

1451030

S3e Cell Sorter, 488 nm and 561 nm 100 mW lasers, 4 fluorescence detectors, with S3e Biosafety System Class I

配套设备

(停产) S3e™ Sheath Cap Assembly, White

1441001

Pkg of 1, white sheath bottle cap assembly with quick disconnect and inline filter, for use with the S3e Cell Sorter

(停产) S3e™ DI Water Cap Assembly, Blue

1441002

Pkg of 1, blue DI water bottle cap assembly with quick disconnect and inline filter, for use with any S3™ or S3e Cell Sorter

(停产) S3e Waste Cap Assembly, Red

1441003

Pkg of 1, red waste bottle cap assembly with quick disconnect, for use with the S3e Cell Sorter

(停产) S3e™ Filter Block A

1441005

Pkg of 1, empty filter block A with 2 empty dichroic filter holders and 3 retaining rings, for use with any S3 or S3e Cell Sorter

(停产) S3e™ Filter Block B

1441006

Pkg of 1, empty filter block B with 2 empty dichroic filter holders and 2 retaining rings, for use with any S3 or S3e Cell Sorter

(停产) S3e™ Individual Filter Holder

1441007

Pkg of 1, empty filter holder with retaining ring, for use with any S3 or S3e Cell Sorter

(停产) Neutral Density Filter 1.0

1441008

Pkg of 1, 1″ neutral density filter, for use with the S3™ cell sorter

(停产) S3e Nozzle Tip, 100 µm

1441009

Pkg of 1, nozzle tip, 100 µm, for use with any S3 or S3e Cell Sorter

(停产) S3e™ Nozzle O-Ring with Alignment Disk

1441010

Pkg of 1, includes 2 nozzle O-rings and 2 alignment disks, for use with any S3 or S3e Cell Sorter

(停产) S3e™ Collection Adaptor Set

1441011

Pkg of 1, collection adapter set, includes 1 right slide holder and 1 left slide holder, for use with the S3 cell sorter

(停产) S3e™ 8-Tube PCR Strip Collection Adaptor Set, low profile

12005068

Pkg of 1, collection adaptor set, includes 1 right and 1 left holder to accommodate low profile 8-tube PCR strips, for use with any S3™ or S3e Cell Sorter

(停产) 2 mm Hex Driver

1441012

Pkg of 1, 2 mm hex driver, for use with any S3™ or S3e™ Cell Sorter

(停产) S3e™ Individual Dichroic Filter Holder

1441013

Pkg of 1, empty dichroic filter holder with retaining ring, for use with any S3 or S3e Cell Sorter

(停产) S3e™ Accessory Kit

1451065

Accessory kit, includes 100 µm nozzle tip, neutral density filter 1.0, spanner wrench, 2 mm hex driver, 1 ml syringe, O-ring, and alignment disk, for use with any S3 or S3e Cell Sorter

Consumables

(停产) ProFlow Cell Filters, sterile, 30 µm, pack of 100

12012576

Pkg of 100, sterile, cup fitting, and disposable cell filters, 30 µm. Used to remove aggregates and to prepare uniform single-cell suspensions from tissue, fluids, plant material, nuclei, etc.

(停产) ProFlow Cell Filters, sterile, 50 µm, pack of 100

12012574

Pkg of 100, sterile, cup fitting, and disposable cell filters.Used to remove aggregates and to prepare uniform single-cell suspensions from tissue, fluids, plant material, nuclei, etc.

(停产) ProFlow Cell Filters, sterile, 70 µm, pack of 100

12012573

Pkg of 100, sterile, cup fitting, and disposable cell filters, 70 µm. Used to remove aggregates and to prepare uniform single-cell suspensions from tissue, fluids, plant material, nuclei, etc.

(停产) ProLine Universal Calibration Beads

1451086

15 ml (3 x 5 ml), ready-to-use universal fluorescent beads for alignment verification and drop delay determination, for use on any S3™ or S3e™ Cell Sorter

(停产) ProLine™ Rainbow Beads

1451085

5 ml (1 x 5 ml), include 3.0–3.4 µm beads dyed with 8 different fluorescent intensities for testing sensitivity and linearity on any S3™ or S3e™ Cell Sorter

(停产) ProLine™ Calibration Beads

1451081

Discontinued Product
This item has been replaced by ProLine Universal Calibration Beads (1451086)

(停产) ProFlow Sort Grade 8x Sheath Fluid

1451082

This product has been replaced by catalog (12012932).
There is no difference in unit volume or composition between the discontinued product and the replacement product.

(停产) ProFlow Sort Grade 8x Sheath Fluid

12012932

20 L (5 x 4 L), ready-to-use, sterile endotoxin- and preservative-free sheath fluid generates 160 L of 1x solution, for use on any S3 or S3e Cell Sorter

(停产) ProFlow™ Sort Grade Water

1451083

20 L (5 x 4 L), ready-to-use, sterile endotoxin-free water in gamma-irradiated S3e Fluidic Containers, for use on any S3 or S3e Cell Sorter

(停产) S3e™ Fluidic Container

1451084

3 x 4 L, gamma-irradiated containers, for use on any S3 or S3e Cell Sorter

下载

ProSort™ Software, Version 1.6, PC (Windows 7 Pro and 10)

Version-16-PC-Windows-7-Pro-and-10

S3e Cell Sorter instrument software. Version 1.6, for PC only (Windows 7 Pro and 10).
Installation instructions for existing users:

Users on V1.5 can go directly to V1.6 installation
Users on V1.4 will download and install V1.5 first (from web), before V1.6 upgrade
For all other ProSort versions, please contact Bio-Rad technical support

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下载

ProSort™ Software, Version 1.5, PC (Windows 7 Pro and 10)

Version-15-PC-Windows-7-Pro-and-10

S3e Cell Sorter instrument software. Version 1.5, for PC only (Windows 7 Pro and 10).