Mouse anti-FES Monoclonal Antibody(1467CT528.22.17)描述别名宿主特异性反应种属应用分子量类型克隆号同种型储存/保存方法研究领域背景说明细胞定位UniProt参考文献
概述 | |
描述 |
Purified Mouse Monoclonal Antibody (Mab)
|
别名 |
FES抗体;Tyrosine-protein kinase Fes/Fps; Feline sarcoma/Fujinami avian sarcoma oncogene homolog; Proto-oncogene c-Fes; Proto-oncogene c-Fps; p93c-fes; FES; FPS
|
宿主 |
Mouse
|
特异性 |
This FES antibody is generated from a mouse immunized with a recombinant protein.
|
反应种属 |
Human
|
应用 |
IHC-P~~1:25
WB~~1:1000 |
分子量 |
Predicted molecular weight: 93kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
性能 | |
类型 |
Monoclonal Antibody
|
克隆号 |
1467CT528.22.17
|
同种型 |
IgG1,k
|
储存/保存方法 |
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
|
研究领域 |
Cancer Antibodies;Microbiology;Signal Transduction;Others
|
靶标 | |
背景说明 |
Tyrosine-protein kinase that acts downstream of cell surface receptors and plays a role in the regulation of the actin cytoskeleton, microtubule assembly, cell attachment and cell spreading. Plays a role in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Acts down- stream of the activated FCER1 receptor and the mast/stem cell growth factor receptor KIT. Plays a role in the regulation of mast cell degranulation. Plays a role in the regulation of cell differentiation and promotes neurite outgrowth in response to NGF signaling. Plays a role in cell scattering and cell migration in response to HGF-induced activation of EZR. Phosphorylates BCR and down-regulates BCR kinase activity. Phosphorylates HCLS1/HS1, PECAM1, STAT3 and TRIM28.
|
细胞定位 |
Cytoplasm, cytosol. Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side Cytoplasmic vesicle. Golgi apparatus. Cell junction, focal adhesion. Note=Distributed throughout the cytosol when the kinase is not activated. Association with microtubules requires activation of the kinase activity. Shuttles between focal adhesions and cell-cell contacts in epithelial cells. Recruited to the lateral cell membrane in polarized epithelial cells by interaction with phosphorylated EZR. Detected at tubular membrane structures in the cytoplasm and at the cell periphery
|
UniProt |
P07332
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参考文献 | |
参考文献 |
Alcalay M.,et al.Oncogene 5:267-275(1990).
Roebroek A.J.M.,et al.EMBO J. 4:2897-2903(1985). Lefebvre J.-C.,et al.Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases. Ota T.,et al.Nat. Genet. 36:40-45(2004). Zody M.C.,et al.Nature 440:671-675(2006). |
实验结果图
All lanes : Anti-FES Antibody at 1:2000 dilution Lane 1: U-251MG whole cell lysates Lane 2: A549 whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 93 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-FES Antibody at 1:1000 dilution + U87-MG whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 93 kDa Blocking/Dilution buffer: 5% NFDM/TBST.